Intracellular delivery of poly(ethylene glycol) conjugated antisense oligonucleotide using cationic lipids by formation of self-assembled polyelectrolyte complex micelles

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A polyelectrolyte complex (PEC) micelle-based antisense oligodeoxynucleotide (ODN) delivery system was designed to overcome intrinsic limitations of cationic lipid-mediated gene transfer. Cationic lipid (Lipofectamine(TM), LF) and ODN conjugated poly(ethylene glycol) (PEG) were ionically complexed to form self-assembled spherical PEC micelles. They have a distinctive structural feature: a charge-neutralized core surrounded by highly flexible PEG corona. The PEC micelles could be visualized as a nano-sized sphere by atomic force microscopy (AFM). The DNA/LF PEC micelles exhibited far improved transfection efficiency compared to those of conventional lipoplex formulations (ODN/LF) in the presence of serum. They showed enhanced cellular uptake followed by rapid nuclear localization of ODN in human epithelial carcinoma (KB) cells. In addition, anti-proliferative activity of c-raf gene-directed antisense ODN was almost fully maintained in KB cells in the presence of serum.
Publisher
Amer Scientific Publishers
Issue Date
2006
Language
English
Article Type
Article
Keywords

GENE DELIVERY; PHYSICOCHEMICAL PROPERTIES; FUSOGENIC PEPTIDE; INHIBITION; LIPOSOMES; SYSTEM; CELLS; OLIGODEOXYNUCLEOTIDES; EXPRESSION; SERUM

Citation

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.6, no.9-10, pp.2790 - 2795

ISSN
1533-4880
URI
http://hdl.handle.net/10203/89235
Appears in Collection
BS-Journal Papers(저널논문)
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