p19(ras) Amplifies p73 beta-induced apoptosis through mitochondrial pathway

Abstract

p73 and p53 have been known to play an important role in cellular damage responses such as apoptosis. Although p73 is a structural and functional homolog of p53 tumor suppressor gene, much less is known about the mechanism of p73-induced apoptotic cell death. In this study, we demonstrate that p19(ras) interaction with p73 beta amplifies p73 beta-induced apoptotic signaling responses including Bax mitochondrial translocation, cytochrome c release, increased production of reactive oxygen species (ROS) and loss of mitochondrial transmembrane potential (Delta psi(m)). Furthermore, endogenous expression of p19(ras) and p73 beta is significantly increased by Taxol treatment, and Taxol-enhanced endogenous p73 beta transcriptional activities are further amplified by p19(ras), which markedly increased cellular apoptosis in p53-null SAOS2 cancer cell line. These results have important implications for understanding the molecular events of p19(ras) to p73 functions in cancer cells. (c) 2008 Elsevier Inc. All rights reserved.