Burkholderia ginsengisoli sp nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field

Abstract

A bacterial strain (designated KMY03(T)) that possesses beta-glucosidase activity was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. The bacterium was found to comprise Gram-negative, rod-shaped, motile cells with unipolar polytrichous flagella. On the basis of 16S rRNA gene sequence similarity, strain KMY03(T) was shown to belong to the family Burkholderiaceae of the Betaproteobacteria, being most closely related to Burkholderia caledonica LMG 19076(T) (97.8%), Burkholderia terricola LIMG 20594(T) (97.5%), Burkholderia xenovorans LMG 21463 T (97.4%) and Burkholderia phytofirmans LMG 22146T (97.3%). Chemotaxonomic data (major ubiquinone, Q-8; major fatty acids, C-17:0 cyclo, C-16:0, C-19:0 cyclo omega 8c and summed feature 2) supported the affiliation of the novel strain with the genus Burkholderia. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed the strain to be differentiated genotypically and phenotypically from Burkholderia species with validly published names. On the basis of these data, strain KMY03(T) represents a novel species of the genus Burkholderia, for which the name Burkholderia ginsengisoli sp. nov. is proposed. The type strain is KMY03(T) (= KCTC 12389(T) = NBRC 100965(T)).