DC Field | Value | Language |
---|---|---|
dc.contributor.author | Reinscheid RK | ko |
dc.contributor.author | Kim, Joon | ko |
dc.contributor.author | Zeng J | ko |
dc.contributor.author | Civelli O | ko |
dc.date.accessioned | 2013-03-06T05:00:01Z | - |
dc.date.available | 2013-03-06T05:00:01Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-09 | - |
dc.identifier.citation | EUROPEAN JOURNAL OF PHARMACOLOGY, v.478, no.1, pp.27 - 34 | - |
dc.identifier.issn | 0014-2999 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85879 | - |
dc.description.abstract | Cyclic adenosine-monophosphate (cAMP) is one of the major second messenger molecules transmitting extracellular stimuli into short-and long-term changes of intracellular homeostasis. Measurements of cellular cAMP levels are often used to quantify and characterize signaling by G protein-coupled receptors. Current assays for cAMP determination are usually end-point assays involving cell lysis. We have developed a technology to monitor real-time changes of cAMP levels in living cells. This method uses a modified cyclic nucleotide-gated (CNG) Ca2+ channel which is opened by intracellular cAMP. Thus, changes in cAMP levels are translated into changes in free Ca2+ which can easily be measured using fluorimetric imaging technologies compatible with high-throughput screening formats. The new assay method was used to characterize the pharmacology of various enclogenously and heterologously expressed G protein-coupled receptors and allows for the simultaneous study of G(s), G(i) and G(q)-linked receptors in the same cell population. (C) 2003 Published by Elsevier B.V. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | LIGAND-BINDING | - |
dc.subject | ORPHANIN-FQ | - |
dc.subject | AMP | - |
dc.subject | EXPRESSION | - |
dc.title | High-throughput real-time monitoring of G(s)-coupled receptor activation in intact cells using cyclic nucleotide-gated channels | - |
dc.type | Article | - |
dc.identifier.wosid | 000185993700004 | - |
dc.identifier.scopusid | 2-s2.0-1642382773 | - |
dc.type.rims | ART | - |
dc.citation.volume | 478 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 27 | - |
dc.citation.endingpage | 34 | - |
dc.citation.publicationname | EUROPEAN JOURNAL OF PHARMACOLOGY | - |
dc.identifier.doi | 10.1016/j.ejphar.2003.08.036 | - |
dc.contributor.localauthor | Kim, Joon | - |
dc.contributor.nonIdAuthor | Reinscheid RK | - |
dc.contributor.nonIdAuthor | Zeng J | - |
dc.contributor.nonIdAuthor | Civelli O | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cyclic nucleotide-gated channel | - |
dc.subject.keywordAuthor | cAMP | - |
dc.subject.keywordAuthor | Ca2+ imaging | - |
dc.subject.keywordAuthor | second messenger assay | - |
dc.subject.keywordPlus | LIGAND-BINDING | - |
dc.subject.keywordPlus | ORPHANIN-FQ | - |
dc.subject.keywordPlus | AMP | - |
dc.subject.keywordPlus | EXPRESSION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.