DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, JS | ko |
dc.contributor.author | Baek, DJ | ko |
dc.contributor.author | Lee, GY | ko |
dc.contributor.author | Kim, YS | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.date.accessioned | 2013-03-06T03:32:30Z | - |
dc.date.available | 2013-03-06T03:32:30Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-01 | - |
dc.identifier.citation | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v.59, pp.166 - 167 | - |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85696 | - |
dc.description.abstract | Malonyl-CoA decarboxylase (MCD), which catalyzes the conversion of malonyl-CoA to acetyl-CoA, is an evolutionarily distinct and highly conserved enzyme. MCD does not share sequence homology with other known decarboxylases, while the enzymes from different species exhibit at least >30% sequence identity to each other. In order to provide a canonical structure of the enzyme for detailed study of its structure-function relationship, the MCD of Rhizobium leguminosarum bv. trifolii was overexpressed and crystallized. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 133.45, b = 127.10, c = 66.37 Angstrom. The asymmetric unit is likely to contain two molecules of MCD (molecular weight of 51 418 Da), with a crystal volume per protein weight (V-M) of 2.69 Angstrom(3) Da(-1) and a solvent content of about 54.3% by volume. A native data set to 3.0 Angstrom resolution was obtained using a rotating-anode X-ray generator. | - |
dc.language | English | - |
dc.publisher | BLACKWELL PUBLISHING | - |
dc.subject | DEFICIENCY | - |
dc.title | Crystallization and preliminary X-ray crystallographic analysis of malonyl-CoA decarboxylase from Rhizobium leguminosarum bv. trifolii | - |
dc.type | Article | - |
dc.identifier.wosid | 000179955400028 | - |
dc.identifier.scopusid | 2-s2.0-0037237633 | - |
dc.type.rims | ART | - |
dc.citation.volume | 59 | - |
dc.citation.beginningpage | 166 | - |
dc.citation.endingpage | 167 | - |
dc.citation.publicationname | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | - |
dc.identifier.doi | 10.1107/S0907444902018905 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Jung, JS | - |
dc.contributor.nonIdAuthor | Baek, DJ | - |
dc.contributor.nonIdAuthor | Lee, GY | - |
dc.contributor.nonIdAuthor | Kim, YS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DEFICIENCY | - |
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