DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Myo-Jeong | ko |
dc.contributor.author | Lee, Hee-Seob | ko |
dc.contributor.author | Cho, Jin-Sook | ko |
dc.contributor.author | Kim, Tae-Jip | ko |
dc.contributor.author | Moon, Tae-Wha | ko |
dc.contributor.author | Oh, Sang-Taek | ko |
dc.contributor.author | Kim, Jung-Wan | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.contributor.author | Park, Kwan-Hwa | ko |
dc.date.accessioned | 2013-03-06T03:12:29Z | - |
dc.date.available | 2013-03-06T03:12:29Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2002-07 | - |
dc.identifier.citation | BIOCHEMISTRY, v.41, no.29, pp.9099 - 9108 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85647 | - |
dc.description.abstract | A novel inhibitor against maltose-producing a-amylase was prepared via stepwise degradation of a high-molecular-weight acarbose (HMWA) using Themus maltogenic amylase (ThMA). The structure of the purified inhibitor was determined to be alpha-D-glucopyranosyl-alpha-acarviosinyl-D-glucopyranose (GlcAcvGlc) by C-13 NMR and MALDI-TOF/MS. Progress curves of PNPG2 hydrolysis by various amylolytic enzymes, including MGase, ThMA, and CDase I-5, in the presence of acarbose or GlcAcvGlc indicated a slow-binding mode of inhibition. Analytical ultracentrifugation and X-ray crystallography analyses revealed that the presence of GlcAcvGlc increased the dimerization of ThMA. The formation of dimer complexed with GlcAcvGlc might induce a conformational change in ThMA, leading to a two-step inhibition process. The inhibition potency of GlcAcvGlc for MGase, ThMA, and CDase I-5 was 3 orders of magnitude higher than that of acarbose. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | THERMOSTABLE MALTOGENIC AMYLASE | - |
dc.subject | X-RAY STRUCTURE | - |
dc.subject | BACILLUS-STEAROTHERMOPHILUS | - |
dc.subject | ENZYMATIC-PROPERTIES | - |
dc.subject | ASPERGILLUS-AWAMORI | - |
dc.subject | ANGSTROM RESOLUTION | - |
dc.subject | BINDING INHIBITOR | - |
dc.subject | ACARBOSE | - |
dc.subject | SLOW | - |
dc.subject | GLUCOAMYLASE | - |
dc.title | Preparation and characterization of alpha-D-glucopyranosyl-alpha-acarviosinyl-D-glucopyranose, a novel inhibitor specific for maltose-producing amylase | - |
dc.type | Article | - |
dc.identifier.wosid | 000176895900012 | - |
dc.identifier.scopusid | 2-s2.0-0037162390 | - |
dc.type.rims | ART | - |
dc.citation.volume | 41 | - |
dc.citation.issue | 29 | - |
dc.citation.beginningpage | 9099 | - |
dc.citation.endingpage | 9108 | - |
dc.citation.publicationname | BIOCHEMISTRY | - |
dc.identifier.doi | 10.1021/bi025586b | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Kim, Myo-Jeong | - |
dc.contributor.nonIdAuthor | Lee, Hee-Seob | - |
dc.contributor.nonIdAuthor | Cho, Jin-Sook | - |
dc.contributor.nonIdAuthor | Kim, Tae-Jip | - |
dc.contributor.nonIdAuthor | Moon, Tae-Wha | - |
dc.contributor.nonIdAuthor | Oh, Sang-Taek | - |
dc.contributor.nonIdAuthor | Kim, Jung-Wan | - |
dc.contributor.nonIdAuthor | Park, Kwan-Hwa | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | THERMOSTABLE MALTOGENIC AMYLASE | - |
dc.subject.keywordPlus | X-RAY STRUCTURE | - |
dc.subject.keywordPlus | BACILLUS-STEAROTHERMOPHILUS | - |
dc.subject.keywordPlus | ENZYMATIC-PROPERTIES | - |
dc.subject.keywordPlus | ASPERGILLUS-AWAMORI | - |
dc.subject.keywordPlus | ANGSTROM RESOLUTION | - |
dc.subject.keywordPlus | BINDING INHIBITOR | - |
dc.subject.keywordPlus | ACARBOSE | - |
dc.subject.keywordPlus | SLOW | - |
dc.subject.keywordPlus | GLUCOAMYLASE | - |
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