DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pinkse, Martijn W. H. | ko |
dc.contributor.author | Maier, Claudia S. | ko |
dc.contributor.author | Kim, Jung-In | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.contributor.author | Heck, Albert J. R. | ko |
dc.date.accessioned | 2013-03-06T03:10:18Z | - |
dc.date.available | 2013-03-06T03:10:18Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-03 | - |
dc.identifier.citation | JOURNAL OF MASS SPECTROMETRY, v.38, no.3, pp.315 - 320 | - |
dc.identifier.issn | 1076-5174 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85640 | - |
dc.description.abstract | The supramolecular assembly of Helicobacter pylori urease was studied by nanoflow electrospray ionization orthogonal time-of-flight mass spectrometry. The measured molecular mass of the urease complex of 1.06 MDa corresponcts to a dodecameric (alphabeta)(12) assembly of urease alpha (26 kDa) and beta (61 kDa) subunits. The dodecamer disassembles readily into (alphabeta)(3) subunits in solution and under controlled collisional-induced dissociation in the gas phase. This is in strong support of an ((alphabeta)(3))(4) architecture consistent with the recently published x-ray structure. In vitro, the alpha and beta subunits are capable of re-assembling to (alphabeta)(3), but not further to.the dodecameric complex. Copyright (C) 2003 John Wiley Sons, Ltd. | - |
dc.language | English | - |
dc.publisher | JOHN WILEY & SONS LTD | - |
dc.subject | APOPROTEIN COMPLEXES | - |
dc.subject | PROTEIN ASSEMBLIES | - |
dc.subject | PURIFICATION | - |
dc.subject | ACTIVATION | - |
dc.subject | SEQUENCE | - |
dc.subject | ORIGIN | - |
dc.subject | UREF | - |
dc.subject | ESI | - |
dc.title | Macromolecular asserably of Helicobacter pylori urease investigated by mass spectrometry | - |
dc.type | Article | - |
dc.identifier.wosid | 000181808900009 | - |
dc.identifier.scopusid | 2-s2.0-0037351309 | - |
dc.type.rims | ART | - |
dc.citation.volume | 38 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 315 | - |
dc.citation.endingpage | 320 | - |
dc.citation.publicationname | JOURNAL OF MASS SPECTROMETRY | - |
dc.identifier.doi | 10.1002/jms.443 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Pinkse, Martijn W. H. | - |
dc.contributor.nonIdAuthor | Maier, Claudia S. | - |
dc.contributor.nonIdAuthor | Kim, Jung-In | - |
dc.contributor.nonIdAuthor | Heck, Albert J. R. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Helicobacter pylori | - |
dc.subject.keywordAuthor | urease | - |
dc.subject.keywordAuthor | protein assembly | - |
dc.subject.keywordAuthor | non-covalent interaction | - |
dc.subject.keywordAuthor | mass spectrometry | - |
dc.subject.keywordPlus | APOPROTEIN COMPLEXES | - |
dc.subject.keywordPlus | PROTEIN ASSEMBLIES | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | ORIGIN | - |
dc.subject.keywordPlus | UREF | - |
dc.subject.keywordPlus | ESI | - |
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