DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, MT | ko |
dc.contributor.author | Hwang, SJ | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-05T03:29:11Z | - |
dc.date.available | 2013-03-05T03:29:11Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-05 | - |
dc.identifier.citation | BIOTECHNOLOGY PROGRESS, v.20, pp.913 - 920 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85274 | - |
dc.description.abstract | Gutless adenoviruses (GAds), namely, all gene-deleted adenoviruses, were developed to minimize their immune responses and toxic effects for a successful gene delivery tool in gene therapy. The Cre/loxP system has been widely used for GAd production. To produce GAd with a low amount of helper adenovirus (HAd) as byproduct, it is indispensable to use 293Cre cells expressing a high level of Cre for GAd production. In this study, we constructed the HAd containing enhanced green fluorescent protein gene flanked by two parallel loxP sites (HAd/GFP). The use of HAd/GFP with flow cytometry allows one to select 293Cre cells expressing a high level of Cre without using conventional Western blot analysis. Unlike conventional HAd titration methods such as plaque assay and end-point dilution assay, it also allows one to monitor rapidly the HAd as byproduct in earlier stages of GAd amplification. Taken together, the use of HAd/GFP with flow cytometry facilitates bioprocess development for efficient GAA production. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | GREEN FLUORESCENT PROTEIN | - |
dc.subject | DEPENDENT VECTOR | - |
dc.subject | IN-VIVO | - |
dc.subject | SYSTEM | - |
dc.subject | EXPRESSION | - |
dc.subject | EXCISION | - |
dc.subject | IMMUNITY | - |
dc.subject | THERAPY | - |
dc.subject | CULTURE | - |
dc.title | Flow cytometric application of helper adenovirus (HAd) containing GFP gene flanked by two parallel loxP sites to evaluation of 293 cre-complementing cell line and monitoring of HAd in gutless Ad production | - |
dc.type | Article | - |
dc.identifier.wosid | 000221841800036 | - |
dc.identifier.scopusid | 2-s2.0-2942739230 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.beginningpage | 913 | - |
dc.citation.endingpage | 920 | - |
dc.citation.publicationname | BIOTECHNOLOGY PROGRESS | - |
dc.identifier.doi | 10.1021/bp034248e | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Park, MT | - |
dc.contributor.nonIdAuthor | Hwang, SJ | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GREEN FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | DEPENDENT VECTOR | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | EXCISION | - |
dc.subject.keywordPlus | IMMUNITY | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | CULTURE | - |
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