Flow cytometric application of helper adenovirus (HAd) containing GFP gene flanked by two parallel loxP sites to evaluation of 293 cre-complementing cell line and monitoring of HAd in gutless Ad production

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dc.contributor.authorPark, MTko
dc.contributor.authorHwang, SJko
dc.contributor.authorLee, Gyun-Minko
dc.date.accessioned2013-03-05T03:29:11Z-
dc.date.available2013-03-05T03:29:11Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2004-05-
dc.identifier.citationBIOTECHNOLOGY PROGRESS, v.20, pp.913 - 920-
dc.identifier.issn8756-7938-
dc.identifier.urihttp://hdl.handle.net/10203/85274-
dc.description.abstractGutless adenoviruses (GAds), namely, all gene-deleted adenoviruses, were developed to minimize their immune responses and toxic effects for a successful gene delivery tool in gene therapy. The Cre/loxP system has been widely used for GAd production. To produce GAd with a low amount of helper adenovirus (HAd) as byproduct, it is indispensable to use 293Cre cells expressing a high level of Cre for GAd production. In this study, we constructed the HAd containing enhanced green fluorescent protein gene flanked by two parallel loxP sites (HAd/GFP). The use of HAd/GFP with flow cytometry allows one to select 293Cre cells expressing a high level of Cre without using conventional Western blot analysis. Unlike conventional HAd titration methods such as plaque assay and end-point dilution assay, it also allows one to monitor rapidly the HAd as byproduct in earlier stages of GAd amplification. Taken together, the use of HAd/GFP with flow cytometry facilitates bioprocess development for efficient GAA production.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.subjectGREEN FLUORESCENT PROTEIN-
dc.subjectDEPENDENT VECTOR-
dc.subjectIN-VIVO-
dc.subjectSYSTEM-
dc.subjectEXPRESSION-
dc.subjectEXCISION-
dc.subjectIMMUNITY-
dc.subjectTHERAPY-
dc.subjectCULTURE-
dc.titleFlow cytometric application of helper adenovirus (HAd) containing GFP gene flanked by two parallel loxP sites to evaluation of 293 cre-complementing cell line and monitoring of HAd in gutless Ad production-
dc.typeArticle-
dc.identifier.wosid000221841800036-
dc.identifier.scopusid2-s2.0-2942739230-
dc.type.rimsART-
dc.citation.volume20-
dc.citation.beginningpage913-
dc.citation.endingpage920-
dc.citation.publicationnameBIOTECHNOLOGY PROGRESS-
dc.identifier.doi10.1021/bp034248e-
dc.contributor.localauthorLee, Gyun-Min-
dc.contributor.nonIdAuthorPark, MT-
dc.contributor.nonIdAuthorHwang, SJ-
dc.type.journalArticleArticle-
dc.subject.keywordPlusGREEN FLUORESCENT PROTEIN-
dc.subject.keywordPlusDEPENDENT VECTOR-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusEXCISION-
dc.subject.keywordPlusIMMUNITY-
dc.subject.keywordPlusTHERAPY-
dc.subject.keywordPlusCULTURE-
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