DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, BS | ko |
dc.contributor.author | Jeong, YT | ko |
dc.contributor.author | Chang, KH | ko |
dc.contributor.author | Kim, JS | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.date.accessioned | 2013-03-05T02:39:02Z | - |
dc.date.available | 2013-03-05T02:39:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-12 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.11, no.6, pp.1087 - 1092 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | http://hdl.handle.net/10203/85085 | - |
dc.description.abstract | The effect of Sodium Butyrate (NaBu) on the N-linked oligosaccharide structure of Erythropoietin (EPO) was investigated. Recombinant human EPO was produced by CHO cells grown in an MEM alpha medium with or without 5 MM NaBu, and purified from the culture supernatants using a heparin-sepharose affinity column and immunoaffinity column. The N-linked oligosaccharides were released enzymatically and isolated by paper chromatography. The isolated oligosaccharides were then labeled with a fluorescent dye, 2-aminobenzamide, and analyzed with MonoQ anion exchange chromatography and GlycosepN amide chromatography for the assignment of a GU (glucose unit) value. A glycan analysis by HPLC showed that the most significant characteristic effect of NaBu was a reduction in the proportion of glycans with tri-and tetrasialylated oligodaccharides from 21.30% (tri-) and 14.86% (tetra-) in the control cultures (without NaBu) to 8.72% (tri-) and 1.25% (tetra-) in the NaBu-treated cultures, respectively. It was also found that the proportion of asialoglycan increased from 12.54% to 23.69% when treated with NaBu. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY BIOTECHNOLOGY | - |
dc.subject | HAMSTER OVARY CELLS | - |
dc.subject | N-LINKED OLIGOSACCHARIDES | - |
dc.subject | SIALIC-ACID | - |
dc.subject | PROTEINS | - |
dc.subject | HEPARIN | - |
dc.subject | BINDING | - |
dc.subject | PURIFICATION | - |
dc.subject | EXPRESSION | - |
dc.title | Effect of Sodium Butyrate on glycosylation of recombinant Erythropoietin | - |
dc.type | Article | - |
dc.identifier.wosid | 000173030500027 | - |
dc.identifier.scopusid | 2-s2.0-0035689844 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 1087 | - |
dc.citation.endingpage | 1092 | - |
dc.citation.publicationname | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.contributor.nonIdAuthor | Chung, BS | - |
dc.contributor.nonIdAuthor | Jeong, YT | - |
dc.contributor.nonIdAuthor | Chang, KH | - |
dc.contributor.nonIdAuthor | Kim, JS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | erythropoietin | - |
dc.subject.keywordAuthor | sodium butyrate | - |
dc.subject.keywordAuthor | glycosylation | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | N-LINKED OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | SIALIC-ACID | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | HEPARIN | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
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