Enhancement of erythropoietin production from Chinese hamster ovary (CHO) cells by introduction of the urea cycle enzymes, carbamoyl phosphate synthetase I and ornithine transcarbamylase

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dc.contributor.authorKim, Nko
dc.contributor.authorLee, Yko
dc.contributor.authorKim, Hko
dc.contributor.authorChoi, Jko
dc.contributor.authorKim, Jko
dc.contributor.authorChang, KHko
dc.contributor.authorKim, Jung Hoeko
dc.contributor.authorKim, HJko
dc.date.accessioned2013-03-04T19:18:02Z-
dc.date.available2013-03-04T19:18:02Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2004-08-
dc.identifier.citationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.14, pp.844 - 851-
dc.identifier.issn1017-7825-
dc.identifier.urihttp://hdl.handle.net/10203/83794-
dc.description.abstractEfficient mammalian erythropoietin (EPO)-expression systems are required for therapeutic applications. The accumulation of ammonia is a major problem in the production of recombinant proteins in cultured animal cells. To counter this problem we introduced the first two genes of the urea cycle, carbamoyl phosphate synthetase (CPSI) and ornithine transcarbamylase (OTC), into IBE Chinese Hamster Ovary (CHO) cells by stable transfection. The resulting cell line, CO5, had a higher growth rate and accumulated less ammonia per cell than the parental cell line, IBE. In addition, it produced 2 times more EPO than the parent, and the purified EPO contained a higher proportion of acidic isoforms with approximately 15% more sialic acid.-
dc.languageEnglish-
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY-
dc.subjectANTIBODY-PRODUCTION-
dc.subjectCONTINUOUS-CULTURE-
dc.subjectHYBRIDOMA CELLS-
dc.subjectBHK CELLS-
dc.subjectAMMONIA-
dc.subjectGROWTH-
dc.subjectEXPRESSION-
dc.subjectGLYCOSYLATION-
dc.subjectMETABOLISM-
dc.subjectOLIGOSACCHARIDES-
dc.titleEnhancement of erythropoietin production from Chinese hamster ovary (CHO) cells by introduction of the urea cycle enzymes, carbamoyl phosphate synthetase I and ornithine transcarbamylase-
dc.typeArticle-
dc.identifier.wosid000223598400030-
dc.identifier.scopusid2-s2.0-4444343170-
dc.type.rimsART-
dc.citation.volume14-
dc.citation.beginningpage844-
dc.citation.endingpage851-
dc.citation.publicationnameJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.contributor.localauthorKim, Jung Hoe-
dc.contributor.nonIdAuthorKim, N-
dc.contributor.nonIdAuthorLee, Y-
dc.contributor.nonIdAuthorKim, H-
dc.contributor.nonIdAuthorChoi, J-
dc.contributor.nonIdAuthorKim, J-
dc.contributor.nonIdAuthorChang, KH-
dc.contributor.nonIdAuthorKim, HJ-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorerythropoietin-
dc.subject.keywordAuthorurea cycle enzymes-
dc.subject.keywordAuthorammonia concentration-
dc.subject.keywordAuthorsialylation-
dc.subject.keywordPlusANTIBODY-PRODUCTION-
dc.subject.keywordPlusCONTINUOUS-CULTURE-
dc.subject.keywordPlusHYBRIDOMA CELLS-
dc.subject.keywordPlusBHK CELLS-
dc.subject.keywordPlusAMMONIA-
dc.subject.keywordPlusGROWTH-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusGLYCOSYLATION-
dc.subject.keywordPlusMETABOLISM-
dc.subject.keywordPlusOLIGOSACCHARIDES-
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