DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, JH | ko |
dc.contributor.author | Park, IY | ko |
dc.contributor.author | Kim, HS | ko |
dc.contributor.author | Lee, WT | ko |
dc.contributor.author | Kim, MS | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.date.accessioned | 2013-03-04T15:42:19Z | - |
dc.date.available | 2013-03-04T15:42:19Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2002-01 | - |
dc.identifier.citation | FASEB JOURNAL, v.16, no.1, pp.429 - 429 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10203/83102 | - |
dc.description.abstract | Parasin I is a potent 19-residue antimicrobial peptide isolated from the skin mucus of wounded catfish (Parasilurus asotus). Here we describe the mechanism of parasin I production from histone H2A in catfish skin mucosa on epidermal injury. Cathepsin D is found to exist in the mucus as an inactive proenzyme (procathepsin D), and a metalloprotease, induced on injury, cleaves procathepsin D to generate active cathepsin D. This activated form of cathepsin D then cleaves the Ser(19)-Arg(20) bond of histone H2A to produce parasin I. Immunohistochemical analysis reveals that unacetylated histone H2A, a precursor of parasin I, and procathepsin D are present in the cytoplasm of epithelial mucous cells and that parasin I is produced on the mucosal surface on epidermal injury. Western blot analysis shows that parasin I is also present in the skin mucus of other fish species. Furthermore, parasin I shows good antimicrobial activity against fish-specific bacterial pathogens. Taken together, these results indicate that cathepsin D and a metalloprotease participate in the production of parasin I from histone H2A and that parasin I contributes to the innate host defense of the fish against invading microorganisms. | - |
dc.language | English | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.subject | MONOCLONAL-ANTIBODIES | - |
dc.subject | PROCATHEPSIN-D | - |
dc.subject | RAINBOW-TROUT | - |
dc.subject | PROTEINS | - |
dc.subject | CATFISH | - |
dc.subject | ANTIBIOTICS | - |
dc.subject | INHIBITORS | - |
dc.subject | IMMUNITY | - |
dc.subject | SEQUENCE | - |
dc.subject | DEFENSE | - |
dc.title | Cathepsin D produces antimicrobial peptide parasin I from histone H2A in the skin mucosa of fish | - |
dc.type | Article | - |
dc.identifier.wosid | 000173656600015 | - |
dc.identifier.scopusid | 2-s2.0-0036514179 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 429 | - |
dc.citation.endingpage | 429 | - |
dc.citation.publicationname | FASEB JOURNAL | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Cho, JH | - |
dc.contributor.nonIdAuthor | Park, IY | - |
dc.contributor.nonIdAuthor | Kim, HS | - |
dc.contributor.nonIdAuthor | Lee, WT | - |
dc.contributor.nonIdAuthor | Kim, MS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | innate immunity | - |
dc.subject.keywordAuthor | metalloprotease | - |
dc.subject.keywordAuthor | Parasilurus asotus | - |
dc.subject.keywordAuthor | teleost | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODIES | - |
dc.subject.keywordPlus | PROCATHEPSIN-D | - |
dc.subject.keywordPlus | RAINBOW-TROUT | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CATFISH | - |
dc.subject.keywordPlus | ANTIBIOTICS | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | IMMUNITY | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | DEFENSE | - |
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