DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yun, Hyungdon | ko |
dc.contributor.author | Lim, Seongyop | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Kim, Byung-Gee | ko |
dc.date.accessioned | 2013-03-04T15:38:12Z | - |
dc.date.available | 2013-03-04T15:38:12Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-04 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.70, no.4, pp.2529 - 2534 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/83094 | - |
dc.description.abstract | Alcaligenes denitrificans Y2k-2 was obtained by selective enrichment followed by screening from soil samples, which showed omega-amino acid:pyruvate transaminase activity, to kinetically resolve aliphatic beta-amino acid, and the corresponding structural gene (aptA) was cloned. The gene was functionally expressed in Escherichia coli BL21 by using an isopropyll-beta-D-thiogalactopyranoside (IPTG)-inducible pET expression system (9.6 U/mg), and the recombinant AptA was purified to show a specific activity of 77.2 U/mg for L-beta-amino-n-butyric acid (L-beta-ABA). The enzyme converts various beta-amino acids and amines to the corresponding beta-keto acids and ketones by using pyruvate as an amine acceptor. The apparent K-m and V-max for L-beta-ABA were 56 mM and 500 U/mg, respectively, in the presence of 10 mM pyruvate. In the presence of 10 mM L-beta-ABA, the apparent K-m and V-max for pyruvate were 11 mM and 370 U/mg, respectively. The enzyme exhibits high stereoselectivity (E > 80) in the kinetic resolution of 50 mM D,L-beta-ABA, producing optically pure D-beta-ABA (99% enantiomeric excess) with 53% conversion. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | COMPLETE GENOME STRUCTURE | - |
dc.subject | ALPHA-METHYLBENZYLAMINE | - |
dc.subject | SEQUENCE | - |
dc.subject | AMINOTRANSFERASES | - |
dc.subject | MICROORGANISMS | - |
dc.subject | INHIBITORS | - |
dc.subject | HOMOLOGY | - |
dc.subject | CLONING | - |
dc.title | omega-amino acid : pyruvate transaminase from Alcaligenes denitrificans Y2k-2: a new catalyst for kinetic resolution of beta-amino acids and amines | - |
dc.type | Article | - |
dc.identifier.wosid | 000220792200079 | - |
dc.identifier.scopusid | 2-s2.0-16544382186 | - |
dc.type.rims | ART | - |
dc.citation.volume | 70 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 2529 | - |
dc.citation.endingpage | 2534 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.identifier.doi | 10.1128/AEM.70.4.2529-2534.2004 | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.nonIdAuthor | Yun, Hyungdon | - |
dc.contributor.nonIdAuthor | Lim, Seongyop | - |
dc.contributor.nonIdAuthor | Kim, Byung-Gee | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | COMPLETE GENOME STRUCTURE | - |
dc.subject.keywordPlus | ALPHA-METHYLBENZYLAMINE | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | AMINOTRANSFERASES | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | HOMOLOGY | - |
dc.subject.keywordPlus | CLONING | - |
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