DC Field | Value | Language |
---|---|---|
dc.contributor.author | Koo, Deog-Bon | ko |
dc.contributor.author | Kang, Yong-Kook | ko |
dc.contributor.author | Park, Jung Sun | ko |
dc.contributor.author | Park, Jin-Ki | ko |
dc.contributor.author | Chang, Won-Kyong | ko |
dc.contributor.author | Lee, Kyung-Kwang | ko |
dc.contributor.author | Han, Yong Mahn | ko |
dc.date.accessioned | 2013-03-04T15:29:03Z | - |
dc.date.available | 2013-03-04T15:29:03Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-09 | - |
dc.identifier.citation | THERIOGENOLOGY, v.62, no.5, pp.779 - 789 | - |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | http://hdl.handle.net/10203/83074 | - |
dc.description.abstract | The structural integrity of blastocyst stage embryos, consisting of the inner cell mass (ICM) and trophectoderm (TE) cells, is a prerequisite for normal development after implantation in mammals. In this study, allocation of nuclear transfer (NT)-derived porcine blastocysts to the ICM and to the TE cells was examined and compared with IVF- and in vivo-derived embryos. NT-derived embryos had a lower developmental competence to the blastocyst stage than IVF-derived embryos (P < 0.05). Total cell number of NT-derived blastocysts was inferior to that of IVF-derived embryos (P < 0.05), although no difference was detected between the two groups in the ratio of ICM to total cells. However, in vivo-derived blastocysts had a higher proportion of ICM to total cells compared with in vitro-produced embryos (P < 0.01). To investigate what proportions of in vitro-produced porcine embryos represent normal structural integrity, differentially-stained blastocysts were individually classified into three presumptive groups (I: < 20%; II: 20-40%; III: > 40%) according to the ratio of ICM to total cells. Low proportions of NT- (12.5%, 7/56) and IVF-derived blastocysts (15.8%, 9/57) were assigned to Group II, presumptively having a normal range of structural integrity, whereas, almost all in vivo-derived embryos (97.5%, 39/40) were allocated to Group II. In conclusion, limited structural integrity may lead to the poor survival to term of NT- or IVF-derived porcine embryos produced in vitro. (c) 2004 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | INNER CELL MASS | - |
dc.subject | NUCLEAR TRANSFER | - |
dc.subject | SOMATIC-CELLS | - |
dc.subject | GENE-TRANSCRIPTION | - |
dc.subject | FETAL FIBROBLASTS | - |
dc.subject | EMBRYOS | - |
dc.subject | PIGS | - |
dc.subject | EXPRESSION | - |
dc.subject | OOCYTES | - |
dc.subject | MOUSE | - |
dc.title | A paucity of structural integrity in cloned porcine blastocysts produced in vitro | - |
dc.type | Article | - |
dc.identifier.wosid | 000227659200001 | - |
dc.identifier.scopusid | 2-s2.0-3142636900 | - |
dc.type.rims | ART | - |
dc.citation.volume | 62 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 779 | - |
dc.citation.endingpage | 789 | - |
dc.citation.publicationname | THERIOGENOLOGY | - |
dc.identifier.doi | 10.1016/j.theriogenology.2003.12.027 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | Koo, Deog-Bon | - |
dc.contributor.nonIdAuthor | Kang, Yong-Kook | - |
dc.contributor.nonIdAuthor | Park, Jung Sun | - |
dc.contributor.nonIdAuthor | Park, Jin-Ki | - |
dc.contributor.nonIdAuthor | Chang, Won-Kyong | - |
dc.contributor.nonIdAuthor | Lee, Kyung-Kwang | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | nuclear transfer | - |
dc.subject.keywordAuthor | in vitro fertilization | - |
dc.subject.keywordAuthor | blastocyst | - |
dc.subject.keywordAuthor | differential staining | - |
dc.subject.keywordAuthor | porcine | - |
dc.subject.keywordPlus | INNER CELL MASS | - |
dc.subject.keywordPlus | NUCLEAR TRANSFER | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | GENE-TRANSCRIPTION | - |
dc.subject.keywordPlus | FETAL FIBROBLASTS | - |
dc.subject.keywordPlus | EMBRYOS | - |
dc.subject.keywordPlus | PIGS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | OOCYTES | - |
dc.subject.keywordPlus | MOUSE | - |
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