DC Field | Value | Language |
---|---|---|
dc.contributor.author | Johnson, Eric T. | ko |
dc.contributor.author | Ryu, Sunhyo | ko |
dc.contributor.author | Yi, Hankuil | ko |
dc.contributor.author | Shin, Byongchul | ko |
dc.contributor.author | Cheong, Hyeonsook | ko |
dc.contributor.author | Choi, Giltsu | ko |
dc.date.accessioned | 2013-03-04T15:19:52Z | - |
dc.date.available | 2013-03-04T15:19:52Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-02 | - |
dc.identifier.citation | PLANT JOURNAL, v.25, no.3, pp.325 - 333 | - |
dc.identifier.issn | 0960-7412 | - |
dc.identifier.uri | http://hdl.handle.net/10203/83050 | - |
dc.description.abstract | Many plant species exhibit a reduced range of flower colors due to the lack of an essential gene or to the substrate specificity of a biosynthetic enzyme. Petunia does not produce orange flowers because dihydroflavonol 4-reductase (DFR) from this species, an enzyme involved in anthocyanin biosynthesis, inefficiently reduces dihydrokaempferol, the precursor to orange pelargonidin-type anthocyanins. The substrate specificity of DFR, however, has not been investigated at the molecular level. By analyzing chimeric DFRs of Petunia and Gerbera, we identified a region that determines the substrate specificity of DFR. Furthermore, by changing a single amino acid in this presumed substrate-binding region, we developed a DFR enzyme that preferentially reduces dihydrokaempferol. Our results imply that the substrate specificity of DFR can be altered by minor changes in DFR. | - |
dc.language | English | - |
dc.publisher | BLACKWELL SCIENCE LTD | - |
dc.subject | UDP-GALACTOSE 4-EPIMERASE | - |
dc.subject | PETUNIA-HYBRIDA | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | FLOWER COLOR | - |
dc.subject | ANTHOCYANIN BIOSYNTHESIS | - |
dc.subject | FLAVONOID BIOSYNTHESIS | - |
dc.subject | ALCOHOL-DEHYDROGENASE | - |
dc.subject | ANGSTROM RESOLUTION | - |
dc.subject | CRYSTAL-STRUCTURES | - |
dc.subject | MOLECULAR-CLONING | - |
dc.title | Alteration of a single amino acid changes the substrate specificity of dihydroflavonol 4-reductase | - |
dc.type | Article | - |
dc.identifier.wosid | 000167007600008 | - |
dc.identifier.scopusid | 2-s2.0-0035115023 | - |
dc.type.rims | ART | - |
dc.citation.volume | 25 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 325 | - |
dc.citation.endingpage | 333 | - |
dc.citation.publicationname | PLANT JOURNAL | - |
dc.identifier.doi | 10.1046/j.1365-313x.2001.00962.x | - |
dc.contributor.localauthor | Choi, Giltsu | - |
dc.contributor.nonIdAuthor | Johnson, Eric T. | - |
dc.contributor.nonIdAuthor | Ryu, Sunhyo | - |
dc.contributor.nonIdAuthor | Yi, Hankuil | - |
dc.contributor.nonIdAuthor | Shin, Byongchul | - |
dc.contributor.nonIdAuthor | Cheong, Hyeonsook | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | dihydroflavonol 4-reductase | - |
dc.subject.keywordAuthor | pelargonidin | - |
dc.subject.keywordAuthor | anthocyanin | - |
dc.subject.keywordAuthor | substrate specificity | - |
dc.subject.keywordAuthor | site-directed mutagenesis | - |
dc.subject.keywordPlus | UDP-GALACTOSE 4-EPIMERASE | - |
dc.subject.keywordPlus | PETUNIA-HYBRIDA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | FLOWER COLOR | - |
dc.subject.keywordPlus | ANTHOCYANIN BIOSYNTHESIS | - |
dc.subject.keywordPlus | FLAVONOID BIOSYNTHESIS | - |
dc.subject.keywordPlus | ALCOHOL-DEHYDROGENASE | - |
dc.subject.keywordPlus | ANGSTROM RESOLUTION | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURES | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
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