DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Jin-Hee | ko |
dc.contributor.author | Lim, Chae-Seok | ko |
dc.contributor.author | Lee, Yong-Seok | ko |
dc.contributor.author | Kandel, Eric R. | ko |
dc.date.accessioned | 2013-03-04T15:03:39Z | - |
dc.date.available | 2013-03-04T15:03:39Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-08 | - |
dc.identifier.citation | LEARNING & MEMORY, v.11, no.4, pp.421 - 435 | - |
dc.identifier.issn | 1072-0502 | - |
dc.identifier.uri | http://hdl.handle.net/10203/83017 | - |
dc.description.abstract | We previously reported that five repeated Pulses of S-HT lead to clown-regulation of the TM-apCAM isoform at the surface of Aplysia sensory neurons (SNs). We here examined whether apCAM down-regulation is required for 5-HT-induced long-term facilitation. We also analyzed the role of the cytoplasmic and extracellular domains by overexpressing various apCAM Mutants by DNA microinjection. When TM-apCAM was up-regulated in SNs by DNA microinjection, five pulses of 5-HT failed to produce either synaptic facilitation or an enhancement of synaptic growth, Suggesting that clown-regulation of apCAM is required for 5-HT-induced EPSP enhancement and new varicosity formation. However, disrupting the extracellular domain function of overexpressed apCAM with a specific antibody restored 5-HT-induced excitatory postsynaptic potential increase but not synaptic growth. The overexpression of the MAP Kinase mutant of TM-apCAM, which is not internalized by 5-HT, inhibited new varicosity formation, but did not inhibit excitatory postsynaptic potential increase. Deletion mutants containing only the cytoplasmic portion of apCAM blocked 5-HT-induced synaptic growth but not excitatory postsynaptic potential increase. Thus, our data Suggest that TM-apCAM may act as a Suppressor of both synaptic-strength enhancement in pre-existing synapses and of new synaptic varicosity formation in the nonsynaptic region, via different mechanisms. | - |
dc.language | English | - |
dc.publisher | Cold Spring Harbor Lab Press | - |
dc.subject | SENSORY NEURONS | - |
dc.subject | HETEROSYNAPTIC FACILITATION | - |
dc.subject | SYNAPTIC PLASTICITY | - |
dc.subject | GILL-WITHDRAWAL | - |
dc.subject | NERVOUS-SYSTEM | - |
dc.subject | NEURITE FASCICULATION | - |
dc.subject | SENSORIMOTOR SYNAPSES | - |
dc.subject | PROTEIN-SYNTHESIS | - |
dc.subject | IN-VITRO | - |
dc.subject | EXPRESSION | - |
dc.title | Role of Aplysia cell adhesion molecules during 5-HT-induced long-term functional and structural changes | - |
dc.type | Article | - |
dc.identifier.wosid | 000223110900009 | - |
dc.identifier.scopusid | 2-s2.0-4043060985 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 421 | - |
dc.citation.endingpage | 435 | - |
dc.citation.publicationname | LEARNING & MEMORY | - |
dc.identifier.doi | 10.1101/lm.61104 | - |
dc.contributor.localauthor | Han, Jin-Hee | - |
dc.contributor.nonIdAuthor | Lim, Chae-Seok | - |
dc.contributor.nonIdAuthor | Lee, Yong-Seok | - |
dc.contributor.nonIdAuthor | Kandel, Eric R. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SENSORY NEURONS | - |
dc.subject.keywordPlus | HETEROSYNAPTIC FACILITATION | - |
dc.subject.keywordPlus | SYNAPTIC PLASTICITY | - |
dc.subject.keywordPlus | GILL-WITHDRAWAL | - |
dc.subject.keywordPlus | NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | NEURITE FASCICULATION | - |
dc.subject.keywordPlus | SENSORIMOTOR SYNAPSES | - |
dc.subject.keywordPlus | PROTEIN-SYNTHESIS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | EXPRESSION | - |
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