DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, J | ko |
dc.contributor.author | Lee, YK | ko |
dc.contributor.author | Kang, BK | ko |
dc.contributor.author | Chung, Won Il | ko |
dc.date.accessioned | 2013-03-04T13:29:24Z | - |
dc.date.available | 2013-03-04T13:29:24Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-09 | - |
dc.identifier.citation | THEORETICAL AND APPLIED GENETICS, v.109, no.8, pp.1562 - 1567 | - |
dc.identifier.issn | 0040-5752 | - |
dc.identifier.uri | http://hdl.handle.net/10203/82784 | - |
dc.description.abstract | A negative selectable marker gene, codA, was successfully co-transformed with a GUS reporter gene to develop selectable marker gene-free transgenic plants. The pNC binary vector contained a T-DNA harboring the codA gene next to the nptII gene, while a second binary vector, pHG, contained a GUS reporter gene. Tobacco plants (Nicotiana tabacum cv. Samsun NN) were co-transformed via the mixture method with Agrobacterium tumefaciens LBA4404 strains harboring pNC and pHG, respectively. Seeds harvested from the co-transformants were sown on germination media containing 5-fluorocytosine (5-FC). Analysis of the progeny by GUS staining and PCR amplification revealed that all of the 5-FC-resistant R-1 plants were codA free, and that the codA gene segregated independently of the GUS gene. Because codA-free seedlings developed normally on 5-FC-containing medium, we suggest that co-transformation with negatively selectable markers is a viable method for the production of easily distinguished, selectable marker gene-free transgenic plants. | - |
dc.language | English | - |
dc.publisher | Springer | - |
dc.subject | T-DNAS | - |
dc.subject | AGROBACTERIUM-TUMEFACIENS | - |
dc.subject | VECTOR SYSTEM | - |
dc.subject | TRANSFORMATION | - |
dc.subject | SELECTION | - |
dc.subject | CYTOCHROME-P450 | - |
dc.subject | SEGREGATION | - |
dc.subject | EXPRESSION | - |
dc.subject | HERBICIDE | - |
dc.subject | CELL | - |
dc.title | Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic plants | - |
dc.type | Article | - |
dc.identifier.wosid | 000225054800002 | - |
dc.identifier.scopusid | 2-s2.0-11144351917 | - |
dc.type.rims | ART | - |
dc.citation.volume | 109 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1562 | - |
dc.citation.endingpage | 1567 | - |
dc.citation.publicationname | THEORETICAL AND APPLIED GENETICS | - |
dc.identifier.doi | 10.1007/s00122-004-1790-x | - |
dc.contributor.localauthor | Chung, Won Il | - |
dc.contributor.nonIdAuthor | Park, J | - |
dc.contributor.nonIdAuthor | Lee, YK | - |
dc.contributor.nonIdAuthor | Kang, BK | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | T-DNAS | - |
dc.subject.keywordPlus | AGROBACTERIUM-TUMEFACIENS | - |
dc.subject.keywordPlus | VECTOR SYSTEM | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | CYTOCHROME-P450 | - |
dc.subject.keywordPlus | SEGREGATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HERBICIDE | - |
dc.subject.keywordPlus | CELL | - |
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