DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yun, Hyungdon | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Kim, Byung-Gee | ko |
dc.date.accessioned | 2013-03-04T13:19:19Z | - |
dc.date.available | 2013-03-04T13:19:19Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-09 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.87, no.6, pp.772 - 778 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/82768 | - |
dc.description.abstract | The kinetic resolution of (R,S) sec-butylamine with the omega-transaminase (TA) from Vibrio fluvialis JS17 was performed under reduced pressure (e.g., 150 torr) to selectively remove an inhibitory product (2-butanone). The evaporation kinetics of 2-butanone at 150 torr in the buffer solution followed the first-order rate law, and the evaporation rate constant was 2.19 1/h, and independent of pH, while the evaporation kinetics of sec-butylamine is dependent on pH. A simple mathematical model of the evaporation of sec-butylamine allowing the estimation of its concentration in the reaction media was developed. The evaporation rate constant of its free amine form and the protonated amine form were 1.00 1/h, and nearly zero, respectively. Although the optimum pH of the omega-TA activity for sec-butylamine is 9.0, the optimal pH of the enzyme reaction under reduced pressure was pH 7.0, due to the higher evaporation rate of sec-butylamine at higher pH above 7.0. Using the recombinant Escherichia coli BL21 overexpressing omega-TA, 400 mM racemic sec-butylamine was resolved successfully to 98% ee of (R)-sec-butylamine with 53% conversion at 150 torr and pH 7.0. (C) 2004 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.publisher | JOHN WILEY SONS INC | - |
dc.subject | ORGANIC-SOLVENTS | - |
dc.subject | ALPHA-METHYLBENZYLAMINE | - |
dc.subject | ENZYMATIC RESOLUTION | - |
dc.subject | MEMBRANE REACTOR | - |
dc.subject | RACEMIC AMINES | - |
dc.subject | CHIRAL AMINES | - |
dc.subject | LIPASE | - |
dc.subject | AMINOTRANSFERASES | - |
dc.subject | TRANSESTERIFICATION | - |
dc.subject | MICROORGANISMS | - |
dc.title | Kinetic resolution of (R,S)-sec-butylamine using omega-transaminase from Vibrio fluvialis JS17 under reduced pressure | - |
dc.type | Article | - |
dc.identifier.wosid | 000223632800009 | - |
dc.identifier.scopusid | 2-s2.0-4644286944 | - |
dc.type.rims | ART | - |
dc.citation.volume | 87 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 772 | - |
dc.citation.endingpage | 778 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.20186 | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.nonIdAuthor | Yun, Hyungdon | - |
dc.contributor.nonIdAuthor | Kim, Byung-Gee | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | chiral amine | - |
dc.subject.keywordAuthor | kinetic resolution | - |
dc.subject.keywordAuthor | omega-transaminase | - |
dc.subject.keywordAuthor | reduced pressure | - |
dc.subject.keywordAuthor | sec-butylamine | - |
dc.subject.keywordPlus | ORGANIC-SOLVENTS | - |
dc.subject.keywordPlus | ALPHA-METHYLBENZYLAMINE | - |
dc.subject.keywordPlus | ENZYMATIC RESOLUTION | - |
dc.subject.keywordPlus | MEMBRANE REACTOR | - |
dc.subject.keywordPlus | RACEMIC AMINES | - |
dc.subject.keywordPlus | CHIRAL AMINES | - |
dc.subject.keywordPlus | LIPASE | - |
dc.subject.keywordPlus | AMINOTRANSFERASES | - |
dc.subject.keywordPlus | TRANSESTERIFICATION | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
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