DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, JJ | ko |
dc.contributor.author | Rho, SH | ko |
dc.contributor.author | Im, YJ | ko |
dc.contributor.author | Kim, Eunjoon | ko |
dc.contributor.author | Eom, SH | ko |
dc.date.accessioned | 2013-03-03T18:46:50Z | - |
dc.date.available | 2013-03-03T18:46:50Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-04 | - |
dc.identifier.citation | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v.57, pp.616 - 617 | - |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.uri | http://hdl.handle.net/10203/79959 | - |
dc.description.abstract | The PSD-95 (postsynaptic density-95) protein, one of the members of the MAGUK (membrane-associated guanylate kinase) family, is composed of three PDZ domains, one SH3 domain and one guanylate kinase-like (GK) domain. The GK domain mediates the scaffolding function of PSD-95 by protein-protein interaction. Here, the GK domain was subcloned, expressed as an intein fusion protein, purified without the intein and then crystallized at room temperature by the hanging-drop vapour-diffusion method using PEG 8000 as a precipitant. The complete native data set was collected to a resolution of 2.35 Angstrom using flash-cooling. The crystals belong to the primitive tetragonal space group P4(3) (or P4(1)), with unit-cell parameters a = b = 70.03 (4), c = 37.64 (1) Angstrom. | - |
dc.language | English | - |
dc.publisher | MUNKSGAARD INT PUBL LTD | - |
dc.subject | TUMOR SUPPRESSOR GENE | - |
dc.subject | POSTSYNAPTIC DENSITY | - |
dc.subject | SH3 DOMAINS | - |
dc.subject | CHANNEL | - |
dc.subject | HOMOLOG | - |
dc.subject | SAP90 | - |
dc.title | Crystallization and preliminary X-ray diffraction studies of the guanylate kinase-like domain of PSD-95 protein from rat | - |
dc.type | Article | - |
dc.identifier.wosid | 000167663500026 | - |
dc.identifier.scopusid | 2-s2.0-0035075718 | - |
dc.type.rims | ART | - |
dc.citation.volume | 57 | - |
dc.citation.beginningpage | 616 | - |
dc.citation.endingpage | 617 | - |
dc.citation.publicationname | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | - |
dc.contributor.localauthor | Kim, Eunjoon | - |
dc.contributor.nonIdAuthor | Kim, JJ | - |
dc.contributor.nonIdAuthor | Rho, SH | - |
dc.contributor.nonIdAuthor | Im, YJ | - |
dc.contributor.nonIdAuthor | Eom, SH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | TUMOR SUPPRESSOR GENE | - |
dc.subject.keywordPlus | POSTSYNAPTIC DENSITY | - |
dc.subject.keywordPlus | SH3 DOMAINS | - |
dc.subject.keywordPlus | CHANNEL | - |
dc.subject.keywordPlus | HOMOLOG | - |
dc.subject.keywordPlus | SAP90 | - |
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