DC Field | Value | Language |
---|---|---|
dc.contributor.author | Van Chi, P | ko |
dc.contributor.author | Truong, Hoang Quoc | ko |
dc.contributor.author | Ha, Nguyen Thuy | ko |
dc.contributor.author | Chung, Won Il | ko |
dc.contributor.author | Binh, Le Tran | ko |
dc.date.accessioned | 2013-03-03T13:58:04Z | - |
dc.date.available | 2013-03-03T13:58:04Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-10 | - |
dc.identifier.citation | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, v.34, no.2, pp.85 - 92 | - |
dc.identifier.issn | 0885-4513 | - |
dc.identifier.uri | http://hdl.handle.net/10203/78968 | - |
dc.description.abstract | We have isolated a genomic clone encoding trichobakin (TBK), a type I ribosome-inactivating protein from the plant Trichosanthes sp. Bac Kan 8-98 (family Cucurbitaceae), by PCR using specific primers designed from the cDNA sequences of alpha -trichosanthin. The sequence encoding mature TBK was constructed in the pET-21d(+) vector for overexpression in Escherichia coli strain BL21(DE3). The recombinant protein was purified to homogeneity by CM-Sepharose chromatography on FPLC with a final yield of about 55 mg/l of culture. The protein has a molecular mass of about 27 kDa, as shown by SDS/PAGE and matrix-assisted laser-desorption ionization MS. It was found that the protein inhibited luciferase mRNA translation in the rabbit reticulocyte cell-free system with an IC50 value (that which causes a 50% reduction of residual translational activity) of about 3.5 pM. The rRNA glycosidase activity of the protein was also proved at the above-mentioned concentration after rRNAs were treated with acid aniline. | - |
dc.language | English | - |
dc.publisher | PORTLAND PRESS | - |
dc.title | Characterization of trichobakin, a type I ribosome-inactivating protein from Trichosanthes sp Bac Kan 8-98 | - |
dc.type | Article | - |
dc.identifier.wosid | 000171916700003 | - |
dc.identifier.scopusid | 2-s2.0-0034769606 | - |
dc.type.rims | ART | - |
dc.citation.volume | 34 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 85 | - |
dc.citation.endingpage | 92 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | - |
dc.identifier.doi | 10.1042/BA20010026 | - |
dc.contributor.localauthor | Chung, Won Il | - |
dc.contributor.nonIdAuthor | Van Chi, P | - |
dc.contributor.nonIdAuthor | Truong, Hoang Quoc | - |
dc.contributor.nonIdAuthor | Ha, Nguyen Thuy | - |
dc.contributor.nonIdAuthor | Binh, Le Tran | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | expression | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordAuthor | RIP | - |
dc.subject.keywordAuthor | rRNA N-glycosidase | - |
dc.subject.keywordAuthor | TBK | - |
dc.subject.keywordPlus | LUFFA-CYLINDRICA | - |
dc.subject.keywordPlus | HIV REPLICATION | - |
dc.subject.keywordPlus | BRYONIA-DIOICA | - |
dc.subject.keywordPlus | MOSAIC-VIRUS | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | INVITRO | - |
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