DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung J.Y. | ko |
dc.contributor.author | Lim S.W. | ko |
dc.contributor.author | Hong Y.J. | ko |
dc.contributor.author | Hwang S.O. | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-03T13:18:58Z | - |
dc.date.available | 2013-03-03T13:18:58Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-03 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.85, no.5, pp.539 - 546 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/78847 | - |
dc.description.abstract | In an attempt to increase the specific thrombopoietin (TPO) productivity (q(TPO)) of recombinant Chinese hamster ovary (rCHO) cells (CHO-TPO), the effect of expression level of calnexin (CNX) and calreticulin (CRT) on qTPO was investigated. To control both CNX and CRT expression levels simultaneously, the Tet-Off system was first introduced in CHO-TPO cells, and stable Tet-Off cells (TPO-Tet-Off) were screened by luciferase assay. The doxycycline-regulated CNX and CRT expression system in rCHO cells (TPO-CNX/CRT) was established by cotransfection of CNX and CRT expression vector and pTK-Hyg vector into TPO-Tet-Off cells and subsequent screening by Western blot analysis of CNX and CRT. The expression levels of CNX and CRT in TPO-CNX/CRT cells could be tightly controlled by adding different concentrations of doxycycline to a culture medium. Compared with the basal level (2 mug/mL doxycyline), a 2.9-fold increase in CNX expression and a 2.8-fold increase in CRT expression were obtained in the absence of doxycycline. This, in turn, resulted in a 1.9-fold increase in q(TPO), not inhibiting cell growth or changing in vivo biological activity of TPO. Taken together, these results demonstrate that a simultaneous overexpression of CNX and CRT can increase the q(TPO) of rCHO cells. (C) 2004 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.publisher | Wiley - V C H Verlag GmbbH & Co. | - |
dc.subject | CHO-CELLS | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | SIALIC-ACID | - |
dc.subject | SECRETION | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | CLONING | - |
dc.subject | CULTURE | - |
dc.subject | OVEREXPRESSION | - |
dc.subject | IDENTIFICATION | - |
dc.title | Effect of Doxycycline-Regulated Calnexin and Calreticulin Expression on Specific Thrombopoietin Productivity of Recombinant Chinese Hamster Ovary Cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000188998800009 | - |
dc.identifier.scopusid | 2-s2.0-1442352043 | - |
dc.type.rims | ART | - |
dc.citation.volume | 85 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 539 | - |
dc.citation.endingpage | 546 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.10919 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Chung J.Y. | - |
dc.contributor.nonIdAuthor | Lim S.W. | - |
dc.contributor.nonIdAuthor | Hong Y.J. | - |
dc.contributor.nonIdAuthor | Hwang S.O. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | calnexin | - |
dc.subject.keywordAuthor | calreticulin | - |
dc.subject.keywordAuthor | Chinese hamster ovary (CHO) cells | - |
dc.subject.keywordAuthor | Tet-Off | - |
dc.subject.keywordAuthor | thrombopoietin | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | SIALIC-ACID | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
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