DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung U. | ko |
dc.contributor.author | Cho Y.S. | ko |
dc.contributor.author | Seong H.M. | ko |
dc.contributor.author | Kim S.J. | ko |
dc.contributor.author | Kim Y.C. | ko |
dc.contributor.author | Chung, An Sik | ko |
dc.date.accessioned | 2013-03-03T09:22:41Z | - |
dc.date.available | 2013-03-03T09:22:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996-03 | - |
dc.identifier.citation | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.29, no.2, pp.111 - 115 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.uri | http://hdl.handle.net/10203/78175 | - |
dc.description.abstract | A novel glutathione S-transferase from Pseudomonas sp. DJ77 was expressed in E. coli and purified by glutathione-affinity chromatography. The enzyme was composed of two identical subunits. The molecular size of the enzyme was 42 kDa by sephadex G-150 gel permeation chromatography and Mr of each subunit was 23 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis. pI value of the enzyme was approximately 5.8 by isoelectric focusing. This enzyme showed the highest activity toward 1-chloro-2,4-dinitrobenzene as the electrophilic substrate. The relative activities toward p-nitrobenzyl chloride and 1,2-dichloro-4-nitrobenzene were 3.8% and 1.3% of the activity toward 1-chloro-2,4-dinitrobenzene, respectively. K-m and V-max values for 1-chloro-2,4-dinitrobenzene calculated by Lineweaver-Burk plot were 0.76 mM and 14.81 mu mol/min/mg, respectively, and those for glutathione were 6.23 mM and 64.93 mu mol/min/mg, respectively. The enzyme showed highest glutathione S-transferase activity at pH 8.0 and was stable between pH 6.0 and 9.0. The enzyme retained its activity up to 35 degrees C for 90 min but was unstable above 45 degrees C. | - |
dc.language | English | - |
dc.publisher | BIOCHEMICAL SOC REPUBLIC KOREA | - |
dc.subject | PROTEUS-MIRABILIS | - |
dc.subject | PURIFICATION | - |
dc.subject | RAT | - |
dc.title | Characterization of a novel glutathione S-transferase from Pseudomonas sp. DJ77 | - |
dc.type | Article | - |
dc.identifier.wosid | A1996UC46000003 | - |
dc.identifier.scopusid | 2-s2.0-0030500103 | - |
dc.type.rims | ART | - |
dc.citation.volume | 29 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 111 | - |
dc.citation.endingpage | 115 | - |
dc.citation.publicationname | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | - |
dc.contributor.nonIdAuthor | Jung U. | - |
dc.contributor.nonIdAuthor | Cho Y.S. | - |
dc.contributor.nonIdAuthor | Seong H.M. | - |
dc.contributor.nonIdAuthor | Kim S.J. | - |
dc.contributor.nonIdAuthor | Kim Y.C. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | glutathione S-transferase | - |
dc.subject.keywordAuthor | kinetic property | - |
dc.subject.keywordAuthor | Pseudomonas | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordPlus | PROTEUS-MIRABILIS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | RAT | - |
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