DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zocher, F | ko |
dc.contributor.author | Krebsfanger, N | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.contributor.author | Bornscheuer, UT | ko |
dc.date.accessioned | 2013-03-03T07:13:32Z | - |
dc.date.available | 2013-03-03T07:13:32Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-09 | - |
dc.identifier.citation | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v.5, no.1-4, pp.199 - 202 | - |
dc.identifier.issn | 1381-1177 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77735 | - |
dc.description.abstract | A recombinant esterase from Pseudomonas fluorescens (PFE) was produced from E. coli cultures and purified to homogeneity resulting in a specific activity of 120 U/mg ( p-nitrophenylacetate assay). PFE is stable in a wide range of pH (6-9) and active from 30-70 degrees C, but rather unstable at temperatures > 50 degrees C. PFE hydrolyzes a wide range of aliphatic and aromatic esters, but no long chain fatty acid esters. The enzyme showed high rate and enantioselectivity in the resolution of alpha-phenylethanol (E > 100) and its acetate (E = 58), while the closely related alpha-phenylpropanol was converted at very low rate and enantioselectivity. 3-Phenylbutyric acid methylester was hydrolyzed at acceptable rate, but low enantioselectivity (E = 3.4-3.7), whereas 2-phenylbutyric acid ethylester was not a substrate for PFE. (C) 1998 Elsevier Science B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | CLONING | - |
dc.subject | GENE | - |
dc.title | Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens | - |
dc.type | Article | - |
dc.identifier.wosid | 000076071300035 | - |
dc.identifier.scopusid | 2-s2.0-0032530178 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 1-4 | - |
dc.citation.beginningpage | 199 | - |
dc.citation.endingpage | 202 | - |
dc.citation.publicationname | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.nonIdAuthor | Zocher, F | - |
dc.contributor.nonIdAuthor | Krebsfanger, N | - |
dc.contributor.nonIdAuthor | Bornscheuer, UT | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordAuthor | enantioselectivity | - |
dc.subject.keywordAuthor | esterase | - |
dc.subject.keywordAuthor | Pseudomonas fluorescens | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | GENE | - |
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