DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, WH | ko |
dc.contributor.author | Kim, EK | ko |
dc.contributor.author | Lee, HB | ko |
dc.contributor.author | Chung, JH | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.date.accessioned | 2013-03-03T07:00:54Z | - |
dc.date.available | 2013-03-03T07:00:54Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996-01 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.18, no.1, pp.57 - 62 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77710 | - |
dc.description.abstract | A gene, aprP, encoding an extracellular alkaline serine protease from a newly isolated Pseudomonas sp. KFCC 10818 was cloned and characterized. Nucleotide sequence analysis revealed an open reading frame of 1,266 nucleotides which could encode a polypeptide comprised of 422 amino acids. The C-terminal 283 residues showed an overall sequence homology with the subtilisin-type serine proteases. When expressed in E. coli, the alkaline protease, AprP, was released to the culture medium. The purified AprP was most active at pH 11. The k(cat)/K-m value of this enzyme was 9.2 x 10(3)S(-1)mM(-1), which is much higher than those of subtilisins. | - |
dc.language | English | - |
dc.publisher | CHAPMAN HALL LTD | - |
dc.subject | ALKALOPHILIC-BACILLUS-SP | - |
dc.subject | SUBTILISIN | - |
dc.subject | PROTEINASE | - |
dc.subject | ENZYME | - |
dc.subject | SEQUENCE | - |
dc.title | Characterization of an alkaline serine protease from an alkaline-resistant Pseudomonas sp: Cloning and expression of the protease gene in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | A1996TR37600011 | - |
dc.identifier.scopusid | 2-s2.0-0030030088 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 57 | - |
dc.citation.endingpage | 62 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.nonIdAuthor | Jang, WH | - |
dc.contributor.nonIdAuthor | Kim, EK | - |
dc.contributor.nonIdAuthor | Lee, HB | - |
dc.contributor.nonIdAuthor | Chung, JH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ALKALOPHILIC-BACILLUS-SP | - |
dc.subject.keywordPlus | SUBTILISIN | - |
dc.subject.keywordPlus | PROTEINASE | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | SEQUENCE | - |
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