DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cha, Hyun-Ju | ko |
dc.contributor.author | Yoon, Hyun-Geun | ko |
dc.contributor.author | Kim, Young-Wan | ko |
dc.contributor.author | Lee, Hee-Seob | ko |
dc.contributor.author | Kim, Jung-Wan | ko |
dc.contributor.author | Kweon, Ki-Sung | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.contributor.author | Park, Kwan-Hwa | ko |
dc.date.accessioned | 2013-03-03T06:42:02Z | - |
dc.date.available | 2013-03-03T06:42:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-04 | - |
dc.identifier.citation | EUROPEAN JOURNAL OF BIOCHEMISTRY, v.253, no.1, pp.251 - 262 | - |
dc.identifier.issn | 0014-2956 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77659 | - |
dc.description.abstract | A gene encoding a maltogenic amylase of Bacillus stearothermophilus ET1 was cloned and expressed in Escherichia coli. DNA sequence analysis indicated that the gene could encode a 69627-Da protein containing 590 amino acids. The predicted amino acid sequence of the enzyme shared 47-70% identity with the sequences of maltogenic amylase from Bacillus licheniformis, neopullulanase from B. stearothermophilus, and cyclodextrin hydrolase (CDase) I-5 from an alkalophilic Bacillus I-5 strain. In addition to starch: pullulan and cyclodextrin, B. stearothermophilus could hydrolyze isopanose, but not panose, to glucose and maltose. Maltogenic amylase hydrolyzed acarbose, a competitive inhibitor of amylases, to glucose and a trisaccharide. When acarbose was incubated with 10% glucose, isoacarbose, containing an alpha-1,6-glucosidic linkage was produced as an acceptor reaction product. B. stearothermophilus maltogenic amylase shared four highly similar regions of amino acids with several amylolytic enzymes. The beta-cyclodextrin-hydrolyzing activity of maltogenic amylase was enhanced to a level equivalent to the activity of CDase when its amino acid sequence between the third and the fourth conserved regions was made more hydrophobic by site-directed mutagenesis. Enhanced transglycosylation activity was observed in most of the mutants. This result suggested that the members of a subfamily of amylolytic enzymes, including maltogenic amylase and CDase, could share similar substrate specificities, enzymatic mechanisms and structure/function relationships. | - |
dc.language | English | - |
dc.publisher | SPRINGER VERLAG | - |
dc.subject | ALPHA-AMYLASE | - |
dc.subject | BACILLUS-STEAROTHERMOPHILUS | - |
dc.subject | THERMOACTINOMYCES-VULGARIS | - |
dc.subject | BRANCHED OLIGOSACCHARIDES | - |
dc.subject | CATALYTIC PROPERTIES | - |
dc.subject | STRUCTURAL FEATURES | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | AMYLOLYTIC ENZYMES | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | GENE | - |
dc.title | Molecular and enzymatic characterization of a maltogenic amylase that hydrolyzes and transglycosylates acarbose | - |
dc.type | Article | - |
dc.identifier.wosid | 000073002500031 | - |
dc.identifier.scopusid | 2-s2.0-0032053544 | - |
dc.type.rims | ART | - |
dc.citation.volume | 253 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 251 | - |
dc.citation.endingpage | 262 | - |
dc.citation.publicationname | EUROPEAN JOURNAL OF BIOCHEMISTRY | - |
dc.identifier.doi | 10.1046/j.1432-1327.1998.2530251.x | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Cha, Hyun-Ju | - |
dc.contributor.nonIdAuthor | Yoon, Hyun-Geun | - |
dc.contributor.nonIdAuthor | Kim, Young-Wan | - |
dc.contributor.nonIdAuthor | Lee, Hee-Seob | - |
dc.contributor.nonIdAuthor | Kim, Jung-Wan | - |
dc.contributor.nonIdAuthor | Kweon, Ki-Sung | - |
dc.contributor.nonIdAuthor | Park, Kwan-Hwa | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Bacillus stearothermophilus | - |
dc.subject.keywordAuthor | maltogenic amylase | - |
dc.subject.keywordAuthor | acarbose | - |
dc.subject.keywordAuthor | transglycosylation | - |
dc.subject.keywordAuthor | site-directed mutagenesis | - |
dc.subject.keywordPlus | ALPHA-AMYLASE | - |
dc.subject.keywordPlus | BACILLUS-STEAROTHERMOPHILUS | - |
dc.subject.keywordPlus | THERMOACTINOMYCES-VULGARIS | - |
dc.subject.keywordPlus | BRANCHED OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | CATALYTIC PROPERTIES | - |
dc.subject.keywordPlus | STRUCTURAL FEATURES | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | AMYLOLYTIC ENZYMES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENE | - |
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