DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Hyun-Soo | ko |
dc.contributor.author | Ha, Nam-Chul | ko |
dc.contributor.author | Kang, Lin-Woo | ko |
dc.contributor.author | Chung, Kyung Min | ko |
dc.contributor.author | Back, Sung Hoon | ko |
dc.contributor.author | Jang, Sung Key | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.date.accessioned | 2013-03-03T06:38:59Z | - |
dc.date.available | 2013-03-03T06:38:59Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-06 | - |
dc.identifier.citation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.273, no.24, pp.15045 - 15052 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77652 | - |
dc.description.abstract | Crystal structure of RNA helicase domain from genotype 1b hepatitis C virus has been determined at 2.3 Angstrom resolution by the multiple isomorphous replacement method. The structure consists of three domains that form a Y-shaped molecule. One is a NTPase domain containing two highly conserved NTP binding motifs. Another is an RNA binding domain containing a conserved RNA binding motif. The third is a helical domain that contains no beta-strand. The RNA binding domain of the molecule is distinctively separated from the other two domains forming an interdomain cleft into which single stranded RNA can be modeled. A channel is found between a pair of symmetry-related molecules which exhibit the most extensive crystal packing interactions. A stretch of single stranded RNA can be modeled with electrostatic complementarity into the interdomain cleft and continuously through the channel. These observations suggest that some form of this dimer is likely to be the functional form that unwinds double stranded RNA processively by passing one strand of RNA through the channel and passing the other strand outside of the dimer. A "descending molecular see-saw" model is proposed that is consistent with directionality of unwinding and other physicochemical properties of RNA helicases. | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | COLI REP HELICASE | - |
dc.subject | NONSTRUCTURAL PROTEIN-3 | - |
dc.subject | NS3 PROTEIN | - |
dc.subject | DNA | - |
dc.subject | BINDING | - |
dc.subject | ENZYME | - |
dc.subject | REPLICATION | - |
dc.subject | SEPARATION | - |
dc.subject | CLEAVAGE | - |
dc.subject | ENCODES | - |
dc.title | Crystal structure of RNA helicase from genotype 1b hepatitis C virus - A feasible mechanism of unwinding duplex RNA | - |
dc.type | Article | - |
dc.identifier.wosid | 000074160400055 | - |
dc.identifier.scopusid | 2-s2.0-0032510963 | - |
dc.type.rims | ART | - |
dc.citation.volume | 273 | - |
dc.citation.issue | 24 | - |
dc.citation.beginningpage | 15045 | - |
dc.citation.endingpage | 15052 | - |
dc.citation.publicationname | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.identifier.doi | 10.1074/jbc.273.24.15045 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Cho, Hyun-Soo | - |
dc.contributor.nonIdAuthor | Ha, Nam-Chul | - |
dc.contributor.nonIdAuthor | Kang, Lin-Woo | - |
dc.contributor.nonIdAuthor | Chung, Kyung Min | - |
dc.contributor.nonIdAuthor | Back, Sung Hoon | - |
dc.contributor.nonIdAuthor | Jang, Sung Key | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | COLI REP HELICASE | - |
dc.subject.keywordPlus | NONSTRUCTURAL PROTEIN-3 | - |
dc.subject.keywordPlus | NS3 PROTEIN | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | ENCODES | - |
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