DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, IY | ko |
dc.contributor.author | Park, CB | ko |
dc.contributor.author | Kim, MS | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.date.accessioned | 2013-03-03T06:20:15Z | - |
dc.date.available | 2013-03-03T06:20:15Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-10 | - |
dc.identifier.citation | FEBS LETTERS, v.437, no.3, pp.258 - 262 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77611 | - |
dc.description.abstract | In response to epidermal injury, Pavasilurus asotus, a catfish, secreted a strong antimicrobial peptide into the epithelial mucosal layer. The molecular mass of the antimicrobial peptide, named parasin I, was 2000.4 Die, as determined by matrix-associated laser desorption ionization mass spectrometry. The complete amino acid sequence of parasin I, which was determined by automated Edman degradation, was Lys-Gly-Arg-Gly-Lys-Gln-Gly-Gly-Lys-Val-Arg-Ala-Lys-Ala-Lys-Thr-Arg-Ser-Ser. Eighteen of the 19 residues ia parasin I sere identical to the N-terminal of buforin I, ct 39-residue antimicrobial peptide derived from the N-terminal of toad histone H2A [Kim et al, (1996) Biochem. Biophys. Res. Commun. 229, 381-387], which implies that parasin I was cleaved off from the N-terminal of catfish histone H2A, Parasin I showed strong antimicrobial activity, about 12-100 times more potent than magainin 2, against a wide spectrum of microorganisms, without any hemolytic activity, Circular dichroism spectra of parasin I indicated a structural content of 11% alpha-helix, 33% beta-sheet, and 56% random coils. The beta-sheet axial projection diagram of parasin I showed an amphipathic structure. Our results indicate that the catfish may produce parasin I from its histone H2A by a specific protease upon injury to protect against invasion by microorganisms. (C) 1998 Federation of European Biochemical Societies. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | XENOPUS-LAEVIS | - |
dc.subject | SKIN | - |
dc.subject | DEFENSINS | - |
dc.subject | CELLS | - |
dc.subject | CDNA | - |
dc.subject | ANTIBIOTICS | - |
dc.subject | LACTOFERRIN | - |
dc.subject | SECRETION | - |
dc.subject | MELITTIN | - |
dc.subject | FRAGMENT | - |
dc.title | Parasin I, an antimicrobial peptide derived from histone H2A in the catfish, Parasilurus asotus | - |
dc.type | Article | - |
dc.identifier.wosid | 000076717700021 | - |
dc.identifier.scopusid | 2-s2.0-0032561422 | - |
dc.type.rims | ART | - |
dc.citation.volume | 437 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 258 | - |
dc.citation.endingpage | 262 | - |
dc.citation.publicationname | FEBS LETTERS | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Park, IY | - |
dc.contributor.nonIdAuthor | Park, CB | - |
dc.contributor.nonIdAuthor | Kim, MS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | antimicrobial peptide | - |
dc.subject.keywordAuthor | histone H2A | - |
dc.subject.keywordAuthor | epidermal injury | - |
dc.subject.keywordAuthor | catfish | - |
dc.subject.keywordAuthor | Parasilurus asotus | - |
dc.subject.keywordPlus | XENOPUS-LAEVIS | - |
dc.subject.keywordPlus | SKIN | - |
dc.subject.keywordPlus | DEFENSINS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CDNA | - |
dc.subject.keywordPlus | ANTIBIOTICS | - |
dc.subject.keywordPlus | LACTOFERRIN | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | MELITTIN | - |
dc.subject.keywordPlus | FRAGMENT | - |
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