DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi J.H. | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.contributor.author | Kim S.Y. | ko |
dc.contributor.author | Oh D.K | ko |
dc.date.accessioned | 2013-03-03T04:45:34Z | - |
dc.date.available | 2013-03-03T04:45:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-06 | - |
dc.identifier.citation | KOREAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.26, no.3, pp.244 - 249 | - |
dc.identifier.issn | 0257-2389 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77283 | - |
dc.description.abstract | An extracellular polysaccharide, methylan, was produced under the specific conditions by Methylobacteriurn organophilum from methanol. The specific growth rate of cells was approximately constant regardless of C/N ratio and the specific product yield was maximum at a C/N ratio of 30. Methylan production was suppressed by the deficiency of mineral ions such as Mn** or Fe** ion. The optimal pH for cell growth and methylan production was 7. Whereas the optimal temperature for cell growth was found to be 37°C, that for methylan production was 30°C. The methanol concentration above 4% completely inhibited the cell growth. The initial methanol concentration for the maximal production of methylan was 0.5% (v/v) and above this concentration, methylan production was markedly inhibited. To overcome the substrate toxicity and inhibition for both cell growth and methylan production, a fed-bach culture of intermittent feeding within 5 g/l methanol was conducted under the optimal culture condition. Methylan production of was stimulated by nitrogen limitation and methylan was accumulated up to 8.7 g/l and cell mass also increased up to 12.4 g/l. | - |
dc.language | English | - |
dc.publisher | Korean Society for Applied Microbiology | - |
dc.title | Optimization of culture conditions for production of a high viscosity polysaccharide, methylan, by methylobacterium organophilum from methanol | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-0031847818 | - |
dc.type.rims | ART | - |
dc.citation.volume | 26 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 244 | - |
dc.citation.endingpage | 249 | - |
dc.citation.publicationname | KOREAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.contributor.nonIdAuthor | Choi J.H. | - |
dc.contributor.nonIdAuthor | Kim S.Y. | - |
dc.contributor.nonIdAuthor | Oh D.K | - |
dc.subject.keywordAuthor | Methanol | - |
dc.subject.keywordAuthor | Methylobacterium organophilum | - |
dc.subject.keywordAuthor | Optimization of culture condition | - |
dc.subject.keywordAuthor | Polysaccharide-methylan | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.