다공성 젤라틴 미립담체를 이용한 동물세포의 배양시 담체 내외부의 세포 분포 및 전단응력의 영향Cell distribution and shear effect on mammalian cell growth in macroporous gelatin microcarriers

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dc.contributor.author임현수ko
dc.contributor.author김정회ko
dc.date.accessioned2013-03-03T04:37:35Z-
dc.date.available2013-03-03T04:37:35Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1995-01-
dc.identifier.citation한국미생물·생명공학회지, v.23, no.5, pp.499 - 505-
dc.identifier.issn1598-642X-
dc.identifier.urihttp://hdl.handle.net/10203/77254-
dc.description.abstractImmobilization of anchorage-dependent animal cells was investigated using macroporous gelatin microcarriers developed in our laboratory. For the observation of the distribution of cells in macroporous beads, Vero-6 cells and CHO cells were cuttured and their distribution in macroporous beads was observed using a confocal microscope. In results, the final concentration of Vero-6 cells and CHO cells on macroporous beads was 2-3 times higher than that on commercial solid microcarriers (Cytodex-3). Also, macroporous microcarriers could hold cells in their macropores. Consequently, the pores protected cells against hydrodynamic shear. Based on Kolmogorov eddy length scale, the smaller eddies (80 $\mu $m) showed the detrimental effect on cells in macroporous beads as compared with 160 $\mu $m of eddies in conventional solid microcarriers.-
dc.languageKorean-
dc.publisher한국미생물·생명공학회-
dc.title다공성 젤라틴 미립담체를 이용한 동물세포의 배양시 담체 내외부의 세포 분포 및 전단응력의 영향-
dc.title.alternativeCell distribution and shear effect on mammalian cell growth in macroporous gelatin microcarriers-
dc.typeArticle-
dc.type.rimsART-
dc.citation.volume23-
dc.citation.issue5-
dc.citation.beginningpage499-
dc.citation.endingpage505-
dc.citation.publicationname한국미생물·생명공학회지-
dc.contributor.localauthor김정회-
dc.contributor.nonIdAuthor임현수-
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