DC Field | Value | Language |
---|---|---|
dc.contributor.author | 전계택 | ko |
dc.contributor.author | 박성관 | ko |
dc.contributor.author | 권호균 | ko |
dc.contributor.author | 정연호 | ko |
dc.contributor.author | 정용섭 | ko |
dc.contributor.author | 장용근 | ko |
dc.contributor.author | 이영행 | ko |
dc.date.accessioned | 2013-03-03T04:11:51Z | - |
dc.date.available | 2013-03-03T04:11:51Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-01 | - |
dc.identifier.citation | KSBB JOURNAL, v.13, no.2, pp.203 - 208 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77163 | - |
dc.description.abstract | We investigated the effects of dissolved oxygen (D.O.) and fructose (C-source) on cell growth and biosynthesis of cyclosporin A (CyA) produced as a secondary metabolite by a wild-type filamentous fungus, Tolypocladium inflatum. This was performed by controlling the level of D.O. and the residual C-source, as required, through adjustment of medium flow rate, medium concentration and agitation rate in fed-batch cultures. CyA production was furned out to be maximal, when D.O. level was controlled around 10% saturated D.O. and concentration of the C-source was maintained sufficiently low (below 2 g/L) not to cause carbon catabolite repression. Under this culture condition, we obtained the highest values of CyA concentration (507.14 mg/L), Qp (2.11 mg CyA/L/hr), $Y_x/s$ (0.49 g DCW/g fructose), $Y_p/s$<(22.56 mg CyA/g fructose), and YTEX>$_p/x$</TEX> (48.31 mg CyA/g DCW), but relatively lower values of cell concentration (11.98 g DCW/L) and cell productivity (0.043 g DCW/L/hr), in comparison with other parallel fed-batch fermentation conditions. These results implied that, in the carbon-limited culture with 10% saturated D.O. level, the producer microorganism utilized the C-source more efficiently for secondary metabolism. | - |
dc.language | Korean | - |
dc.publisher | 한국생물공학회 | - |
dc.title | 용존산소 제한 또는 탄소원 제한 조건의 유가식배양에서의 Cyclosporin A 생합성 연구 | - |
dc.title.alternative | Studies of Cyclosporin A Biosynthesis under the Conditions of Limited Dissolved Oxygen or Carbon Source in Fed-batch Culture | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 203 | - |
dc.citation.endingpage | 208 | - |
dc.citation.publicationname | KSBB JOURNAL | - |
dc.contributor.localauthor | 장용근 | - |
dc.contributor.nonIdAuthor | 전계택 | - |
dc.contributor.nonIdAuthor | 박성관 | - |
dc.contributor.nonIdAuthor | 권호균 | - |
dc.contributor.nonIdAuthor | 정연호 | - |
dc.contributor.nonIdAuthor | 정용섭 | - |
dc.contributor.nonIdAuthor | 이영행 | - |
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