This paper presents an accurate algorithm for geometric calibration of X-ray imaging system. Calibration is a very important process for improving an imaging system performance. There has been a lot of previous works using linear camera modeling technique, where lens distortion is neglected and/or center of distortion is assumed to be known. Geometrical distortion of image intensifier, however, is very large and its center of distortion should be calculated. This paper presents a new calibration method to estimate the intensifier position and orientation, scale factor, distortion coefficient, magnification factor, and center of distortion using the least square method. We investigate the properties of the algorithm by computer simulation. Simulation results show that the parameters can be estimated accurately using the proposed algorithm.