DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, JH | ko |
dc.contributor.author | Bae, SH | ko |
dc.contributor.author | Choi, Byong-Seok | ko |
dc.date.accessioned | 2013-03-02T20:42:36Z | - |
dc.date.available | 2013-03-02T20:42:36Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-04 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.97, no.9, pp.4591 - 4596 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/75435 | - |
dc.description.abstract | In contrast to the highly mutagenic pyrimidine(6-4)pyrimidone photoproduct, its Dewar valence isomer (Dewar product) has low mutagenic potential and produces a broad range of mutations [LeClerc, J. E., Borden. A. & Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88. 9685-9689]. To determine the origin of the mutagenic property of the Dewar product, we used experimental NMR restraints and molecular dynamics to determine the solution structure of a Dewar-lesion DNA decamer duplex. This DNA decamer duplex (DW/GA duplex) contains a mismatched base pair between the 3' T residue of the Dewar lesion (T6) and an opposed G residue (G15). The 3' T (T6) of the Dewar lesion formed stable hydrogen bonds with the opposing G15 residue. However, the helical bending and unwinding angles of the DW/GA duplex were much larger than those of a second duplex that contains the Dewar lesion and opposing A15 and A16 residues (DW/AA duplex). The DW/GA duplex showed poorer stacking interactions at the two bases of the Dewar product and at the adjacent A7.T14 base pair than did the DW/AA duplex. These structural features imply that no thermal stability or conformational benefit is obtained by incorporating a G instead of an A opposite the 3' T of the Dewar lesion. These properties may thus facilitate the preferential incorporation of an A in accordance with the A rule during translesion replication and lead to the low frequency of 3' T-->C mutations observed at this site. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | RELAXATION MATRIX REFINEMENT | - |
dc.subject | THYMINE CYCLOBUTANE DIMER | - |
dc.subject | DNA DECAMER DUPLEX | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | SKIN-CANCER | - |
dc.subject | CIS-SYN | - |
dc.subject | DEOXYRIBONUCLEIC-ACID | - |
dc.subject | APYRIMIDINIC SITES | - |
dc.subject | 6-4 PHOTOPRODUCT | - |
dc.subject | ABASIC SITE | - |
dc.title | The Dewar photoproduct of thymidylyl(3 -> 5 )-thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule" | - |
dc.type | Article | - |
dc.identifier.wosid | 000086703000038 | - |
dc.identifier.scopusid | 2-s2.0-0034712856 | - |
dc.type.rims | ART | - |
dc.citation.volume | 97 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 4591 | - |
dc.citation.endingpage | 4596 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.contributor.localauthor | Choi, Byong-Seok | - |
dc.contributor.nonIdAuthor | Lee, JH | - |
dc.contributor.nonIdAuthor | Bae, SH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RELAXATION MATRIX REFINEMENT | - |
dc.subject.keywordPlus | THYMINE CYCLOBUTANE DIMER | - |
dc.subject.keywordPlus | DNA DECAMER DUPLEX | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SKIN-CANCER | - |
dc.subject.keywordPlus | CIS-SYN | - |
dc.subject.keywordPlus | DEOXYRIBONUCLEIC-ACID | - |
dc.subject.keywordPlus | APYRIMIDINIC SITES | - |
dc.subject.keywordPlus | 6-4 PHOTOPRODUCT | - |
dc.subject.keywordPlus | ABASIC SITE | - |
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