DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, SJ | ko |
dc.contributor.author | Choi, SY | ko |
dc.contributor.author | Cho, SW | ko |
dc.contributor.author | Choe, Joonho | ko |
dc.contributor.author | Jang, SH | ko |
dc.contributor.author | Song, MS | ko |
dc.contributor.author | Lee, BR | ko |
dc.contributor.author | Park, JS | ko |
dc.date.accessioned | 2013-03-02T19:10:37Z | - |
dc.date.available | 2013-03-02T19:10:37Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996-03 | - |
dc.identifier.citation | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.9, no.4, pp.112 - 117 | - |
dc.identifier.uri | http://hdl.handle.net/10203/75076 | - |
dc.description.abstract | Succinic semialdehyde reductase was inactivated by o-phthalaldehyde. The inactivation followed pseudo-first order kinetics, and the second-order rate constant for the inactivation process was 28 M^(-1)s^(-1) at pH 7.4 and 2590. The absorption spectrum (λ_(max) 337 nm) and fluorescence excitation (λ_(max) 340 nm) and fluorescence emission spectra (λ_(max) 409 nm) were consistent with the formation of an isoindole derivative in the catalytic site between a cysteine and a lysine residue approximately about 3 Å apart. The substrate, succinic semialdehyde, did not protect enzymatic activity against inactivation, whereas the coenzyme NADPH protected against o-phthaladehyde induced inactivation of the enzyme. About 1 isoindole group per ㏖ of the enzyme was formed following complete loss of enzymatic activity. These results suggest that the amino acid residues of the enzyme participating in a reaction with o-phthalaldehyde are cysteinyl and lysyl residues at or near the NADPH binding site. | - |
dc.language | English | - |
dc.publisher | 한국생화학회 | - |
dc.title | Inactivation of Brain Succinic Semialdehyde Reductase by o - Phthaldehyde | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 112 | - |
dc.citation.endingpage | 117 | - |
dc.citation.publicationname | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | - |
dc.contributor.localauthor | Choe, Joonho | - |
dc.contributor.nonIdAuthor | Lee, SJ | - |
dc.contributor.nonIdAuthor | Choi, SY | - |
dc.contributor.nonIdAuthor | Cho, SW | - |
dc.contributor.nonIdAuthor | Jang, SH | - |
dc.contributor.nonIdAuthor | Song, MS | - |
dc.contributor.nonIdAuthor | Lee, BR | - |
dc.contributor.nonIdAuthor | Park, JS | - |
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