cAMP response element-binding protein-binding protein binds to human papillomavirus E2 protein and activates E2-dependent transcription

Abstract

cAMP response element-binding protein-binding protein (CBP) is a eucaryotic transcriptional co-activator that contains multiple protein-protein interaction domains for association with various transcription factors, components of the basal transcriptional apparatus, and other co-activator proteins, Here, we report that CBP is also a co-activator of the human papillomavirus (HPV) E2 protein, which is a sequence-specific transcription/replication factor. We provide biochemical, genetic, and functional evidence that CBP binds directly to HPV E2 in vivo and in vitro and activates Ea-dependent transcription. Mutations in an amphipathic helix within HPV-18 E2 abolish its transcriptional activation properties and its ability to bind to CBP, Furthermore, the binding of CBP to E2 was shown to be necessary for Ea-dependent transcription. Interestingly, the histone acetyltransferase activity of CBP plays a role in CBP activation of Ea-dependent transcription.