Characterization of the enzymatic properties of the yeast Dna2 helicase/endonuclease suggests a new model for Okazaki fragment processing

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dc.contributor.authorBae, Sung-Hoko
dc.contributor.authorSeo, Yeon-Sooko
dc.date.accessioned2013-03-02T18:04:03Z-
dc.date.available2013-03-02T18:04:03Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2000-12-
dc.identifier.citationJOURNAL OF BIOLOGICAL CHEMISTRY, v.275, no.48, pp.38022 - 38031-
dc.identifier.issn0021-9258-
dc.identifier.urihttp://hdl.handle.net/10203/74830-
dc.description.abstractThe Saccharomyces cerevisiae Dna2, which contains single-stranded DNA-specific endonuclease activity, interacts genetically and physically with Fen-l, a structure-specific endonuclease implicated in Okazaki fragment maturation during lagging strand synthesis. In this report, we investigated the properties of the Dna2 helicase/endonuclease activities in search of their in vivo physiological functions in eukaryotes. We found that the Dnaa helicase activity translocates in the 5' to 3' direction and uses DNA with free ends as the preferred substrate. Furthermore, the endonucleolytic cleavage activity of Dna2 was markedly stimulated, by the presence of an RNA segment at the 5'-end of single-stranded DNA and occurred within the DNA, ensuring the complete removal of the initiator RNA segment on the Okazaki fragment. In addition, we demonstrated that the removal of pre-existing initiator 5'-terminal RNA segments depended on a displacement reaction carried out during the DNA polymerase delta -catalyzed elongation of the upstream Okazaki fragments. These properties indicate that Dna2 is well suited to remove the primer RNA on the Okazaki fragment. Based op this information, we propose a new model in which Dna2 plays a direct role in Okazaki fragment maturation in conjunction with Fen-l.-
dc.languageEnglish-
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC-
dc.subjectBASE EXCISION-REPAIR-
dc.subjectSINGLE-STRANDED-DNA-
dc.subjectPROKARYOTIC RNASE HII-
dc.subjectREPLICATION FACTOR-C-
dc.subjectSACCHAROMYCES-CEREVISIAE-
dc.subjectSV40 ORIGIN-
dc.subjectSCHIZOSACCHAROMYCES-POMBE-
dc.subjectENDONUCLEASE ACTIVITY-
dc.subjectHELICASE-
dc.subjectRAD27-
dc.titleCharacterization of the enzymatic properties of the yeast Dna2 helicase/endonuclease suggests a new model for Okazaki fragment processing-
dc.typeArticle-
dc.identifier.wosid000165618700096-
dc.identifier.scopusid2-s2.0-0034528196-
dc.type.rimsART-
dc.citation.volume275-
dc.citation.issue48-
dc.citation.beginningpage38022-
dc.citation.endingpage38031-
dc.citation.publicationnameJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.identifier.doi10.1074/jbc.M006513200-
dc.contributor.localauthorSeo, Yeon-Soo-
dc.contributor.nonIdAuthorBae, Sung-Ho-
dc.type.journalArticleArticle-
dc.subject.keywordPlusBASE EXCISION-REPAIR-
dc.subject.keywordPlusSINGLE-STRANDED-DNA-
dc.subject.keywordPlusPROKARYOTIC RNASE HII-
dc.subject.keywordPlusREPLICATION FACTOR-C-
dc.subject.keywordPlusSACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusSV40 ORIGIN-
dc.subject.keywordPlusSCHIZOSACCHAROMYCES-POMBE-
dc.subject.keywordPlusENDONUCLEASE ACTIVITY-
dc.subject.keywordPlusHELICASE-
dc.subject.keywordPlusRAD27-
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