DC Field | Value | Language |
---|---|---|
dc.contributor.author | Suk, Kyoungho | ko |
dc.contributor.author | Hwang, Dae-Yeon | ko |
dc.contributor.author | Kim, Sunshin | ko |
dc.contributor.author | Kim, Yun-Hee | ko |
dc.contributor.author | Kim, Kyoung Ah | ko |
dc.contributor.author | Seo, Yeon-Soo | ko |
dc.contributor.author | Suh, Yeon-Lim | ko |
dc.contributor.author | Lee, Seong Beom | ko |
dc.contributor.author | Namkung, Namkunge | ko |
dc.contributor.author | Shin, Hee-Sup | ko |
dc.contributor.author | Lee, Myung-Shik | ko |
dc.date.accessioned | 2013-03-02T18:00:24Z | - |
dc.date.available | 2013-03-02T18:00:24Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-09 | - |
dc.identifier.citation | DIABETES RESEARCH AND CLINICAL PRACTICE, v.50, no.1, pp.17 - 26 | - |
dc.identifier.issn | 0168-8227 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74821 | - |
dc.description.abstract | Recently, IA-2, one of Ihs major diabetic autoantigens, and PTP35 cDNA were independently isolated by subtraction cloning using insulinoma cells and a polymerase chain reaction (PCR)-based search for conserved sequences using NIH3T3 fibroblast cell line, respectively. By Southern blot analysis and nucleotide sequence determination of reverse transcription PCR products, we showed that IA-2 and PTP35 are identical and exist as a single gene in a mouse genome. The expression of IA-2/PTP35 messages was detected by northern blot analysis in MIN6N8 cells, an insulinoma cell line derived from non-obese diabetic mice, but its expression level was not affected by the ambient glucose level, phorbol-12-myristate 13-acetate or tumour necrosis factor-alpha. We also generated polyclonal antibodies to murine IA-2/PTP35 by immunization with recombinant proteins. Subsequent immunohistochemical analysis using those polyclonal antibodies disclosed that IA-2/PTP35 is strongly expressed in mouse neuroendocrine tissues such as pancreatic islets and the hypothalamus-pituitary gland. These results suggest that IA-2/PTP35 functions primarily in neuroendocrine tissues. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI IRELAND LTD | - |
dc.subject | DEPENDENT DIABETES-MELLITUS | - |
dc.subject | TRANSMEMBRANE PROTEIN | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | AUTOANTIGEN | - |
dc.subject | IDENTIFICATION | - |
dc.subject | IA-2-BETA | - |
dc.subject | CELLS | - |
dc.subject | AUTOANTIBODIES | - |
dc.subject | INSULINOMA | - |
dc.subject | SEQUENCE | - |
dc.title | Identity of mouse IA-2 and PTP35 genes of the tyrosine phosphatase family, and their expression in neuroendocrine tissues | - |
dc.type | Article | - |
dc.identifier.wosid | 000089020900002 | - |
dc.identifier.scopusid | 2-s2.0-0033879425 | - |
dc.type.rims | ART | - |
dc.citation.volume | 50 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 17 | - |
dc.citation.endingpage | 26 | - |
dc.citation.publicationname | DIABETES RESEARCH AND CLINICAL PRACTICE | - |
dc.identifier.doi | 10.1016/S0168-8227(00)00162-5 | - |
dc.contributor.localauthor | Seo, Yeon-Soo | - |
dc.contributor.nonIdAuthor | Suk, Kyoungho | - |
dc.contributor.nonIdAuthor | Hwang, Dae-Yeon | - |
dc.contributor.nonIdAuthor | Kim, Sunshin | - |
dc.contributor.nonIdAuthor | Kim, Yun-Hee | - |
dc.contributor.nonIdAuthor | Kim, Kyoung Ah | - |
dc.contributor.nonIdAuthor | Suh, Yeon-Lim | - |
dc.contributor.nonIdAuthor | Lee, Seong Beom | - |
dc.contributor.nonIdAuthor | Namkung, Namkunge | - |
dc.contributor.nonIdAuthor | Shin, Hee-Sup | - |
dc.contributor.nonIdAuthor | Lee, Myung-Shik | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | IA-2 | - |
dc.subject.keywordAuthor | PTP35 | - |
dc.subject.keywordAuthor | autoimmune diabetes | - |
dc.subject.keywordAuthor | neuroendocrine tissues | - |
dc.subject.keywordPlus | DEPENDENT DIABETES-MELLITUS | - |
dc.subject.keywordPlus | TRANSMEMBRANE PROTEIN | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | AUTOANTIGEN | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | IA-2-BETA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | AUTOANTIBODIES | - |
dc.subject.keywordPlus | INSULINOMA | - |
dc.subject.keywordPlus | SEQUENCE | - |
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