DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Moon-Ju | ko |
dc.contributor.author | Cha, Sun-Shin | ko |
dc.contributor.author | Park, Jong-Hyeok | ko |
dc.contributor.author | Cha, Hyun-Ju | ko |
dc.contributor.author | Lee, Hee-Seob | ko |
dc.contributor.author | Park, Kwan-Hwa | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.date.accessioned | 2013-03-02T16:47:02Z | - |
dc.date.available | 2013-03-02T16:47:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-05 | - |
dc.identifier.citation | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v.54, pp.416 - 418 | - |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74521 | - |
dc.description.abstract | A novel maltogenic amylase from Bacillus stearothermophilus ET1, which has a dual activity of alpha-1,4- and alpha-1,6-glycosidic bond cleavages and alpha-1,6-glycosidic bond formation, was crystallized by using the hanging-drop vapor-diffusion method. The best crystals were obtained by employing a high concentration of protein (56 mg ml(-1)) and a precipitant containing 22% glycerol, 1.6 M ammonium sulfate in 0.1 M Tris-HCl (pH 8.5). Native diffraction data to 2.66 Angstrom resolution have been obtained from crystals flash-frozen at 110 K. The crystals belong to the space group P2(1)2(1)2(1) pith unit-cell dimensions of a = 77.62, b = 121.23, c = 244.29 Angstrom, and contain three or four protomers per asymmetric unit. Structure determination by multiple isomorphous replacement is in progress. | - |
dc.language | English | - |
dc.publisher | MUNKSGAARD INT PUBL LTD | - |
dc.subject | PANCREATIC ALPHA-AMYLASE | - |
dc.subject | AWAMORI VAR X100 | - |
dc.subject | 2.2-ANGSTROM RESOLUTION | - |
dc.subject | BETA-AMYLASE | - |
dc.subject | MOLECULAR REPLACEMENT | - |
dc.subject | ANGSTROM RESOLUTION | - |
dc.subject | REFINED STRUCTURE | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | ACTIVE-SITE | - |
dc.subject | GLUCOAMYLASE | - |
dc.title | Preliminary X-ray crystallographic analysis of a novel maltogenic amylase from Bacillus stearothermophilus ET1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000073827200019 | - |
dc.identifier.scopusid | 2-s2.0-0032077165 | - |
dc.type.rims | ART | - |
dc.citation.volume | 54 | - |
dc.citation.beginningpage | 416 | - |
dc.citation.endingpage | 418 | - |
dc.citation.publicationname | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | - |
dc.identifier.doi | 10.1107/S0907444997011736 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Cho, Moon-Ju | - |
dc.contributor.nonIdAuthor | Cha, Sun-Shin | - |
dc.contributor.nonIdAuthor | Park, Jong-Hyeok | - |
dc.contributor.nonIdAuthor | Cha, Hyun-Ju | - |
dc.contributor.nonIdAuthor | Lee, Hee-Seob | - |
dc.contributor.nonIdAuthor | Park, Kwan-Hwa | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PANCREATIC ALPHA-AMYLASE | - |
dc.subject.keywordPlus | AWAMORI VAR X100 | - |
dc.subject.keywordPlus | 2.2-ANGSTROM RESOLUTION | - |
dc.subject.keywordPlus | BETA-AMYLASE | - |
dc.subject.keywordPlus | MOLECULAR REPLACEMENT | - |
dc.subject.keywordPlus | ANGSTROM RESOLUTION | - |
dc.subject.keywordPlus | REFINED STRUCTURE | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | ACTIVE-SITE | - |
dc.subject.keywordPlus | GLUCOAMYLASE | - |
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