Heat inducible expression of the CDC70 gene under the control of heat shock element in Saccharomyces cerevisiae

Abstract

In order to express the CDC70 gene of Saccharomyces cerevisiae by heat shock, we have designed heat inducibe hybrid promoters using the Drosophila melanogaster heat shock elements (HSEs), A 220-bp-long upstream fragment of the D. melanogaster hsp70 gene comprised of four HSEs was placed upstream of the putative proximal TATA box of the CDC70 gene, Hybrid promoters containing different fusion joints were tested for their ability to drive the CDC70 gene expression by heat shock The results showed that the HSEs of D, melanogaster conferred the heat-induced CDC70 gene expression, but the heat inducibility was much lower than that in D. melanogaster.