Expression of C5 protein, the protein component of Escherichia coli RNase P from the tac promoter

Cited 13 time in webofscience Cited 0 time in scopus
  • Hit : 374
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorPark, BHko
dc.contributor.authorChoi, YNko
dc.contributor.authorPark, JWko
dc.contributor.authorSim, Sko
dc.contributor.authorGil, MCko
dc.contributor.authorKim, Sko
dc.contributor.authorKim, Mko
dc.contributor.authorLee, Younghoonko
dc.date.accessioned2013-03-02T14:34:25Z-
dc.date.available2013-03-02T14:34:25Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1998-02-
dc.identifier.citationMOLECULES AND CELLS, v.8, no.1, pp.96 - 100-
dc.identifier.issn1016-8478-
dc.identifier.urihttp://hdl.handle.net/10203/74004-
dc.description.abstractThe accurate function of C5 protein, the protein component of Escherichia coil RNase P, is uncertain in vivo. A controllable expression system for C5 protein was constructed which can be used to investigate effects of C5 protein on various cellular functions including biosynthesis of RNase P in vivo. The semisynthetic rnpA gene encoding C5 protein was fused to the tac promoter of the pKK223-3 expression vector. This tac promoter expression system produced a high level of C5 protein upon induction with isopropyl-beta-D-thiogalacto-pyranoside. When the overexpressed C5 protein was purified and used for reconstitution of RNase P, the reconstituted enzyme was active, The N-terminal amino acid of the overexpressed C5 protein was leucine specified by the second codon of the rnpA gene. The more controllable expression system was constructed by introducing the lacI(q) gene into the vector sequence itself.-
dc.languageEnglish-
dc.publisherSPRINGER-VERLAG SINGAPORE PTE LTD-
dc.subjectRIBONUCLEASE-P-
dc.subjectSEQUENCES-
dc.subjectGENE-
dc.subjectINITIATION-
dc.subjectBINDING-
dc.subjectCLONING-
dc.subjectVECTORS-
dc.subjectSITES-
dc.titleExpression of C5 protein, the protein component of Escherichia coli RNase P from the tac promoter-
dc.typeArticle-
dc.identifier.wosid000072500600014-
dc.identifier.scopusid2-s2.0-0032573995-
dc.type.rimsART-
dc.citation.volume8-
dc.citation.issue1-
dc.citation.beginningpage96-
dc.citation.endingpage100-
dc.citation.publicationnameMOLECULES AND CELLS-
dc.contributor.localauthorLee, Younghoon-
dc.contributor.nonIdAuthorPark, BH-
dc.contributor.nonIdAuthorChoi, YN-
dc.contributor.nonIdAuthorPark, JW-
dc.contributor.nonIdAuthorSim, S-
dc.contributor.nonIdAuthorGil, MC-
dc.contributor.nonIdAuthorKim, S-
dc.contributor.nonIdAuthorKim, M-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorC5 protein-
dc.subject.keywordAuthoroverexpression-
dc.subject.keywordAuthorRNase P-
dc.subject.keywordAuthorrnpA-
dc.subject.keywordAuthortac promoter-
dc.subject.keywordPlusRIBONUCLEASE-P-
dc.subject.keywordPlusSEQUENCES-
dc.subject.keywordPlusGENE-
dc.subject.keywordPlusINITIATION-
dc.subject.keywordPlusBINDING-
dc.subject.keywordPlusCLONING-
dc.subject.keywordPlusVECTORS-
dc.subject.keywordPlusSITES-
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 13 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0