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College of Life Science and Bioengineering(생명과학기술대학)Dept. of Biological Sciences(생명과학과)BS-Journal Papers(저널논문)

High level secretion of recombinant staphylokinase into periplasm of Escherichia coli

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dc.contributor.authorLee, Sang Junko
dc.contributor.authorKim, Il Chulko
dc.contributor.authorKim, Dong Minko
dc.contributor.authorBae, Kwang Heeko
dc.contributor.authorByun, Si Myungko
dc.date.accessioned2013-02-28T06:21:14Z-
dc.date.available2013-02-28T06:21:14Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1998-
dc.identifier.citationBIOTECHNOLOGY LETTERS, v.20, no.2, pp.113 - 116-
dc.identifier.issn0141-5492-
dc.identifier.urihttp://hdl.handle.net/10203/73217-
dc.description.abstractThe staphylokinase (SAK) gene from Staphylococcus aureus NCTC10033 was inserted into an expression vector, pKK-ompA, having a tac promoter and an ompA signal sequence. Escherichia coli JM109 carrying the recombinant plasmid produced and secreted the recombinant SAK (rSAK) at 15ug/ml into periplasm and 5ug/ml to extracellular media, respectively. The rSAK was purified with 59% yield by simple procedures from the periplasm of E. coli. The aminoterminal sequence and human plasminogen activating activity of rSAK were coincided with the authentic SAK.-
dc.languageEnglish-
dc.publisherSpringer-
dc.subjectEXPRESSION-
dc.subjectSTREPTOKINASE-
dc.subjectMECHANISM-
dc.subjectCLONING-
dc.subjectGENE-
dc.titleHigh level secretion of recombinant staphylokinase into periplasm of Escherichia coli-
dc.typeArticle-
dc.identifier.wosid000072035000004-
dc.identifier.scopusid2-s2.0-0031884604-
dc.type.rimsART-
dc.citation.volume20-
dc.citation.issue2-
dc.citation.beginningpage113-
dc.citation.endingpage116-
dc.citation.publicationnameBIOTECHNOLOGY LETTERS-
dc.contributor.nonIdAuthorLee, Sang Jun-
dc.contributor.nonIdAuthorKim, Il Chul-
dc.contributor.nonIdAuthorKim, Dong Min-
dc.contributor.nonIdAuthorBae, Kwang Hee-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusSTREPTOKINASE-
dc.subject.keywordPlusMECHANISM-
dc.subject.keywordPlusCLONING-
dc.subject.keywordPlusGENE-
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