Differential roles of the 5' untranslated regions of cucumber mosaic virus RNA 1, 2, 3 and 4 in mRNA competition

Cited 8 time in webofscience Cited 0 time in scopus
  • Hit : 353
  • Download : 0
RNA species of plant tripartite RNA viruses show distinct translational activities in vitro when the Viral RNA concentration is high. However, it is not known what causes the differential translation of virion RNAs. Using an in vitro wheat germ translation system, we investigated the translation efficiencies and competitive activities of chimeric cucumber mosaic virus (CMV) RNAs that contained viral untranslated regions (UTRs) and a luciferase-coding sequence. The chimeric RNAs exhibited distinct translation efficiencies and competitive activities. For example, the translation of chimeric CMV RNA 4 was about 40-fold higher than that of chimeric CMV RNA 3 in a competitive environment. The distinct translation resulted mainly from differences in competitive activities rather than translation efficiencies of the chimeric RNAs. The differential competitive activities were specified by viral 5' UTRs, but not by 3' UTRs or viral proteins. The competitive translational activities of the 5' UTRs were as follows: RNA 4 (coat protein) > RNAs 2 and 1 (2a and 1a protein, or replicase) > RNA 3 (3a protein). (C) 2000 Elsevier Science B.V. All. rights reserved.
Publisher
Elsevier Science Bv
Issue Date
2000-06
Language
English
Article Type
Article
Keywords

EUKARYOTIC MESSENGER-RNAS; SECONDARY STRUCTURE; PROTEIN; REPLICATION; INITIATION; INVITRO; MODULATE; SYSTEM

Citation

VIRUS RESEARCH, v.66, no.2, pp.175 - 185

ISSN
0168-1702
URI
http://hdl.handle.net/10203/72664
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 8 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0