DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Si Hyoung | ko |
dc.contributor.author | Kim, Il Chul | ko |
dc.contributor.author | Lee, Weon Sup | ko |
dc.contributor.author | Byun, Si Myung | ko |
dc.date.accessioned | 2013-02-28T01:00:40Z | - |
dc.date.available | 2013-02-28T01:00:40Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996-06 | - |
dc.identifier.citation | FEMS MICROBIOLOGY LETTERS, v.139, no.2-3, pp.189 - 193 | - |
dc.identifier.issn | 0378-1097 | - |
dc.identifier.uri | http://hdl.handle.net/10203/71876 | - |
dc.description.abstract | The introduction of a plasmid containing skc (streptokinase-coding gene) fused with ompA signal sequence into Escherichia coli K-12 strains, rendered the bacteria mucoid, Measurement of the synthesis of beta-galactosidase from a cps-lacZ fusion (lacZ fusion to a gene necessary for capsule synthesis) showed that the mucoid phenotype was due to induction of the capsular polysaccharide colanic acid synthesis. The introduction of a plasmid carrying skc fused with malE (gene encoding maltose-binding protein) also induced cps-lacZ expression, but intracellular expression of streptokinase in E. coli did not. The cps expression by secretion of streptokinase was diminished to the basal level in a cps-lacZ strain carrying a rcsC mutation. These results show that the secretion of streptokinase in E. coli induces colanic acid synthesis through the RcsC-dependent pathway. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | CAPSULAR POLYSACCHARIDE SYNTHESIS | - |
dc.subject | CLONING | - |
dc.subject | GENE | - |
dc.subject | REGULATOR | - |
dc.subject | IDENTIFICATION | - |
dc.subject | SEQUENCES | - |
dc.subject | STRAINS | - |
dc.subject | INVIVO | - |
dc.title | RcsC-mediated induction of colanic acid by secretion of streptokinase in Escherichia coli K-12 | - |
dc.type | Article | - |
dc.identifier.wosid | A1996UU42000017 | - |
dc.type.rims | ART | - |
dc.citation.volume | 139 | - |
dc.citation.issue | 2-3 | - |
dc.citation.beginningpage | 189 | - |
dc.citation.endingpage | 193 | - |
dc.citation.publicationname | FEMS MICROBIOLOGY LETTERS | - |
dc.identifier.doi | 10.1111/j.1574-6968.1996.tb08201.x | - |
dc.contributor.nonIdAuthor | Lee, Si Hyoung | - |
dc.contributor.nonIdAuthor | Kim, Il Chul | - |
dc.contributor.nonIdAuthor | Lee, Weon Sup | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
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