DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, J.H. | ko |
dc.contributor.author | Kim, G.J. | ko |
dc.contributor.author | Lee, S.G. | ko |
dc.contributor.author | Lee, D.C. | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2013-02-27T23:19:33Z | - |
dc.date.available | 2013-02-27T23:19:33Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-01 | - |
dc.identifier.citation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.81, no.1, pp.53 - 65 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.uri | http://hdl.handle.net/10203/71449 | - |
dc.description.abstract | A thermostable D-hydantoinase was isolated from thermophilic Bacillus thermoatenalatus GH-2 and purified to homogeneity by using immunoaffinity chromatography. The molecular mass of the enzyme was determined to be about 230 kDa, and a value of 56 kDa was obtained as a molecular mass of the subunit on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, implying that oligomeric structure of the enzyme is tetrameric. Isoelectric pH of the enzyme was found to be approx 4.3. The enzyme required Mn2+ for the activity and exhibited its highest activity with phenylhydantoin as a substrate. The optimal pH and temperature for catalytic activity were about 7.5 and 65 degrees C, respectively. The half-life of the enzyme was estimated to be about 45 min at 80 degrees C. | - |
dc.language | English | - |
dc.publisher | HUMANA PRESS INC | - |
dc.subject | MICROBIAL TRANSFORMATION | - |
dc.subject | AMINO-ACIDS | - |
dc.subject | STEAROTHERMOPHILUS SD-1 | - |
dc.subject | ENZYME | - |
dc.subject | MICROORGANISMS | - |
dc.subject | EXPRESSION | - |
dc.subject | CLONING | - |
dc.subject | GENE | - |
dc.title | Purification and characterization of thermostable D-hydantoinase from Bacillus thermocatenulatus GH-2 | - |
dc.type | Article | - |
dc.identifier.wosid | 000082748700005 | - |
dc.identifier.scopusid | 2-s2.0-0032873779 | - |
dc.type.rims | ART | - |
dc.citation.volume | 81 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 53 | - |
dc.citation.endingpage | 65 | - |
dc.citation.publicationname | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Park, J.H. | - |
dc.contributor.nonIdAuthor | Kim, G.J. | - |
dc.contributor.nonIdAuthor | Lee, S.G. | - |
dc.contributor.nonIdAuthor | Lee, D.C. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | thermostability | - |
dc.subject.keywordAuthor | D-hydantoinase | - |
dc.subject.keywordAuthor | Bacillus | - |
dc.subject.keywordAuthor | immunoaffinity | - |
dc.subject.keywordPlus | MICROBIAL TRANSFORMATION | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | STEAROTHERMOPHILUS SD-1 | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | GENE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.