DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, YJ | ko |
dc.contributor.author | Han, SH | ko |
dc.contributor.author | Kang, JS | ko |
dc.contributor.author | Koh, WS | ko |
dc.contributor.author | Yang, Kyu Hwan | ko |
dc.date.accessioned | 2013-02-27T16:43:42Z | - |
dc.date.available | 2013-02-27T16:43:42Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-02 | - |
dc.identifier.citation | TOXICOLOGY LETTERS, v.104, no.3, pp.195 - 202 | - |
dc.identifier.issn | 0378-4274 | - |
dc.identifier.uri | http://hdl.handle.net/10203/69659 | - |
dc.description.abstract | The mechanism by which 2-acetylaminofluorene (AAF) inhibited nitric oxide (NO) formation, in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was investigated. The decrease in NO, as demonstrated by measurement of nitrite was found to correlate well with a decrease in inducible nitric oxide synthase (iNOS) mRNA. Since the promoter in iNOS gene contains binding motifs for NF-kappa B/Rel, AP-1, and NF-IL6 which appear to be important for LPS-mediated iNOS induction, the effect of AAF on the activation of these transcription factors was determined. Treatment of AAF to RAW 264.7 cells induced a dose-related inhibition of NF-kappa B/IRel in chloramphenicol acetyltransferase activity, while either AP-1 or NF-IL6 activation was not affected by AAF. Treatment of RAW 264.7 cells with AAF inhibited protein/DNA binding of NF-kappa B/Rel to its cognate site as measured by electrophoretic mobility shift assay. In addition, AAF treatment caused a significant reduction of nuclear c-rel, p65, and p50 protein levels, and this decrease was paralleled by the accumulation of cytoplasmic c-rel, p65, and p50. These data suggest that AAF inhibits iNOS gene expression by a mechanism involving a blockade of LPS-induced nuclear translocation of NF-kappa B/Rel. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI IRELAND LTD | - |
dc.subject | INTERLEUKIN-2 RECEPTOR EXPRESSION | - |
dc.subject | INTERFERON-GAMMA | - |
dc.subject | MURINE SPLENOCYTES | - |
dc.subject | VIRAL REPLICATION | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | SYNTHASE GENE | - |
dc.subject | L-ARGININE | - |
dc.subject | 2-ACETYLAMINOFLUORENE | - |
dc.subject | INDUCTION | - |
dc.subject | LIPOPOLYSACCHARIDE | - |
dc.title | Acetylaminofluorene inhibits nitric oxide production in LPS-stimulated RAW 264.7 cells by blocking NF-kappa B/Rel activation | - |
dc.type | Article | - |
dc.identifier.wosid | 000079008200004 | - |
dc.type.rims | ART | - |
dc.citation.volume | 104 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 195 | - |
dc.citation.endingpage | 202 | - |
dc.citation.publicationname | TOXICOLOGY LETTERS | - |
dc.identifier.doi | 10.1016/S0378-4274(98)00372-5 | - |
dc.contributor.nonIdAuthor | Jeon, YJ | - |
dc.contributor.nonIdAuthor | Han, SH | - |
dc.contributor.nonIdAuthor | Kang, JS | - |
dc.contributor.nonIdAuthor | Koh, WS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | 2-acetylaminofluorene | - |
dc.subject.keywordAuthor | inducible nitric oxide synthase | - |
dc.subject.keywordAuthor | nuclear factor-kappa B/Rel | - |
dc.subject.keywordPlus | INTERLEUKIN-2 RECEPTOR EXPRESSION | - |
dc.subject.keywordPlus | INTERFERON-GAMMA | - |
dc.subject.keywordPlus | MURINE SPLENOCYTES | - |
dc.subject.keywordPlus | VIRAL REPLICATION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | SYNTHASE GENE | - |
dc.subject.keywordPlus | L-ARGININE | - |
dc.subject.keywordPlus | 2-ACETYLAMINOFLUORENE | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
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