Organization and nucleotide sequence of genes for hemagglutinin components of Clostridium botulinum type B progenitor toxin

Abstract

The genes for hemagglutinin components (33 kD, 17 kD, and 21.5 kD) of Clostridium botulinum type B progenitor toxin were cloned and sequenced. Analysis of the nucleotide sequence showed that the 33 kD, 17 kD, and 21.5 kD hemagglutinin genes were organized into an operon in the 5' upstream region of the toxin gene and their ORF orientation were opposite to that of the toxin gene. A comparison of amino acid sequences between the hemagglutinin components in type B and type C progenitor toxin showed significant homology. Northern blot analysis also revealed that all of the genes for the hemagglutinin components were transcribed as a polycistronic RNA.