DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, EY | ko |
dc.contributor.author | Kim, KA | ko |
dc.contributor.author | Kim, YD | ko |
dc.contributor.author | Kwon, DH | ko |
dc.contributor.author | Lee, HS | ko |
dc.contributor.author | Kim, CH | ko |
dc.contributor.author | Chung, TW | ko |
dc.contributor.author | Byun, Si Myung | ko |
dc.date.accessioned | 2013-02-27T16:18:20Z | - |
dc.date.available | 2013-02-27T16:18:20Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-02 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.22, no.3, pp.201 - 204 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/69553 | - |
dc.description.abstract | Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N-acetylglucosaminyltransferase-III. With the new procedure, human N-acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold. | - |
dc.language | English | - |
dc.publisher | KLUWER ACADEMIC PUBL | - |
dc.title | A simplified procedure for the purification of human N-acetylgucosaminyl-transferase-Ⅲ from human hepatocellular carcinoma tissues | - |
dc.type | Article | - |
dc.identifier.wosid | 000085121200006 | - |
dc.type.rims | ART | - |
dc.citation.volume | 22 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 201 | - |
dc.citation.endingpage | 204 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.identifier.doi | 10.1023/A:1005641115696 | - |
dc.contributor.nonIdAuthor | Song, EY | - |
dc.contributor.nonIdAuthor | Kim, KA | - |
dc.contributor.nonIdAuthor | Kim, YD | - |
dc.contributor.nonIdAuthor | Kwon, DH | - |
dc.contributor.nonIdAuthor | Lee, HS | - |
dc.contributor.nonIdAuthor | Kim, CH | - |
dc.contributor.nonIdAuthor | Chung, TW | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | hepatocellular carcinoma | - |
dc.subject.keywordAuthor | immunoaffinity column | - |
dc.subject.keywordAuthor | monoclonal antibody | - |
dc.subject.keywordAuthor | N-acetylglucosaminyl-transferase | - |
dc.subject.keywordPlus | CDNA CLONING | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SERUM | - |
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