DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, YH | ko |
dc.contributor.author | Bae, KH | ko |
dc.contributor.author | Kim, TJ | ko |
dc.contributor.author | Park, KH | ko |
dc.contributor.author | Lee, HS | ko |
dc.contributor.author | Byun, Si Myung | ko |
dc.date.accessioned | 2013-02-27T15:39:36Z | - |
dc.date.available | 2013-02-27T15:39:36Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1997-02 | - |
dc.identifier.citation | BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, v.41, no.2, pp.227 - 234 | - |
dc.identifier.issn | 1039-9712 | - |
dc.identifier.uri | http://hdl.handle.net/10203/69397 | - |
dc.description.abstract | Cyclodextrin glycosyltransferase (CGTase; EC 2.4.1.19) catalyzes the degradation of starch into cyclodextrins through an intramolecular transglycosylation reaction. Tyr-89, Asn-94, and Tyr-100 are located near the putative active center. To analyze their roles in product specificity, Tyr-89, Asn-94, and Tyr-100 of CGTase from alkalophilic Bacillus sp. I-5 were replaced with different amino acids. Among the mutants, the N94S mutant protein produced about two times more alpha-cyclodextrin than the wild-type at all incubation times. The Y89F and Y100F mutant proteins were changed to more beta-specific enzymes. From these results it is suggested that the changing of the residues located at the near active site can change the product specificity of CGTase. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS AUST | - |
dc.subject | CYCLOMALTODEXTRIN GLUCANOTRANSFERASE | - |
dc.subject | ALPHA-CYCLODEXTRIN | - |
dc.subject | CLONING | - |
dc.title | Effect on product specificity of cyclodextrin glycosyltransferase by site-directed mutagenesis | - |
dc.type | Article | - |
dc.identifier.wosid | A1997WM10300002 | - |
dc.identifier.scopusid | 2-s2.0-0031428432 | - |
dc.type.rims | ART | - |
dc.citation.volume | 41 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 227 | - |
dc.citation.endingpage | 234 | - |
dc.citation.publicationname | BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | - |
dc.contributor.nonIdAuthor | Kim, YH | - |
dc.contributor.nonIdAuthor | Bae, KH | - |
dc.contributor.nonIdAuthor | Kim, TJ | - |
dc.contributor.nonIdAuthor | Park, KH | - |
dc.contributor.nonIdAuthor | Lee, HS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CYCLOMALTODEXTRIN GLUCANOTRANSFERASE | - |
dc.subject.keywordPlus | ALPHA-CYCLODEXTRIN | - |
dc.subject.keywordPlus | CLONING | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.