DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, YM | ko |
dc.contributor.author | Park, Chan Beum | ko |
dc.contributor.author | Lee, SB | ko |
dc.date.accessioned | 2013-02-27T15:36:53Z | - |
dc.date.available | 2013-02-27T15:36:53Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.5, no.1, pp.53 - 56 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/69387 | - |
dc.description.abstract | A thermostable esterase from the hyperthermophilic archaeon Sulfolobus solfataricus was partially purified 590-fold with 16.2% recovery. The partially purified esterase had a specific activity of 29.5 μmol min mg when the enzyme activity was determined using p-nitrophenyl butyrate as a substrate. The apparent molecular weight was about 100 kDa, while the optimum temperature and pH for esterase were 75°C and 8.0, respectively. The enzyme showed high thermal stability and solvent tolerance in comparison to its mesophilic counterpart. The enzyme also showed chiral resolution activity for (S)-ibuprofen, indicating that S. solfataricus esterase can be used for the production of commercially important chiral drugs. | - |
dc.language | Korean | - |
dc.publisher | Korean Society for Biotechnology and Bioengineering | - |
dc.title | Partial purification and characterization of thermostable esterase from the hyperthermophilic archaeon Sulfolobus solfataricus | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-33645620298 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 53 | - |
dc.citation.endingpage | 56 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.contributor.localauthor | Park, Chan Beum | - |
dc.contributor.nonIdAuthor | Chung, YM | - |
dc.contributor.nonIdAuthor | Lee, SB | - |
dc.subject.keywordAuthor | Ibuprofen resolution | - |
dc.subject.keywordAuthor | S. solfataricus | - |
dc.subject.keywordAuthor | Thermostable esterase | - |
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