DNA-binding and enhancer-blocking domains of the Drosophila suppressor of Hairy-wing protein

Cited 54 time in webofscience Cited 0 time in scopus
  • Hit : 315
  • Download : 0
Mutations in the suppressor of Hairy-wing [su(Hw)] gene of Drosophila melanogaster can cause female sterility and suppress mutations that are insertions of the gypsy retrotransposon. Gypsy binds the protein (SUHW) encoded by su(Hw), and SUHW prevents enhancers promoter-distal to gypsy from activating gene transcription. SUHW contains 12 zinc fingers flanked by acidic N- and C-terminal domains. We examined the roles of each of the 12 zinc fingers in binding gypsy DNA and classified them into four groups: essential (fingers 6 through 10); beneficial but nonessential (fingers 1, 2, 3, and 11); unimportant (fingers 5 and 12); and inhibitory (finger 4). Because finger 10 is not required for female fertility but is essential for binding gypsy, these results imply that the SUHW-binding sites required for oogenesis differ in sequence from the gypsy-binding sites. We also examined the functions of the N- and C-terminal domains of SUHW by determining the ability of various deletion mutants to support female fertility and to alter expression of gypsy insertion alleles of the yellow, cut, forked, and Utrabithorax genes. No individual segment of the N- and C-terminal domains of SUHW is absolutely required to alter expression of gypsy insertion alleles. However, the most important domain lies between residues 737 and 880 in the C-terminal domain. This region also contains the residues required for female fertility, and the fertility domain may be;congruent with the enhancer-blocking domain. These results imply that SUHW blocks different enhancers and supports oogenesis by the same or closely related molecular mechanisms.
Publisher
AMER SOC MICROBIOLOGY
Issue Date
1996-07
Language
English
Article Type
Article
Keywords

INDIVIDUAL ZINC FINGERS; TRANSCRIPTION FACTOR-IIIA; INTERNAL CONTROL REGION; 5S RNA GENE; BITHORAX COMPLEX; MELANOGASTER-SUPPRESSOR; CRYSTAL-STRUCTURE; LEUCINE-ZIPPER; CUT LOCUS; EXPRESSION

Citation

MOLECULAR AND CELLULAR BIOLOGY, v.16, no.7, pp.3381 - 3392

ISSN
0270-7306
URI
http://hdl.handle.net/10203/68044
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 54 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0