DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최준호 | ko |
dc.contributor.author | 정해원 | ko |
dc.contributor.author | 조철오 | ko |
dc.date.accessioned | 2013-02-27T07:34:10Z | - |
dc.date.available | 2013-02-27T07:34:10Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1990-01 | - |
dc.identifier.citation | BMC REPORTS, v.23, no.8, pp.53 - 57 | - |
dc.identifier.issn | 1125-8687 | - |
dc.identifier.uri | http://hdl.handle.net/10203/67277 | - |
dc.description.abstract | Enzyme activities of DNA polymerase α and β have been analyzed in anti (+)-r-7, t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE) treated mouse sarcoma cells. The time courses of repair synthesis following treatment of mouse sarcoma cells with 400 ng/ml of BPDE as measured by ³H-thymidine incorporation into the cells for 1 hr show biphasic curve, which indicates two apparent repair phases. Two distinct chromatographic elution profiles of DNA polymerases were observed when cells were treated with carcinogen and post incubated for 3 or 9 hrs, and cell homogenates were applied onto DEAE-52 anion exchange column. Cellular level of DNA polymerase α activity allowed to repair the DNA damage for 3 hrs was significantly higher than that of DNA polymerase β while DNA polymerase β activity was a predominant one in 9 hrs post incubated cells. Data presented here suggest that both DNA polymerase α and β are required to resynthesize the excised DNA damage, and that DNA polymerase α is the major enzyme involved in the resynthesis of DNA damage after endonuclease nicking step while DNA polymerase β is playing the major role in filling the rest of the gap thereafter. | - |
dc.language | Korean | - |
dc.publisher | 생화학분자생물학회 | - |
dc.title | Benzo(a)pyrene Diolepoxide를 처리한 쥐 육종세포내의 DNA Polymerase α and β의 활성도의 변화 | - |
dc.title.alternative | Cellular Levels of DNA Polymerase α and β Activities in Mouse Sarcoma Cells Trated with Benzo(a)pyrene Diolepoxide | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 53 | - |
dc.citation.endingpage | 57 | - |
dc.citation.publicationname | BMC REPORTS | - |
dc.contributor.localauthor | 최준호 | - |
dc.contributor.localauthor | 조철오 | - |
dc.contributor.nonIdAuthor | 정해원 | - |
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