DC Field | Value | Language |
---|---|---|
dc.contributor.author | LEE, JY | ko |
dc.contributor.author | ROH, JR | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2013-02-27T07:29:16Z | - |
dc.date.available | 2013-02-27T07:29:16Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1994-05 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.43, no.11, pp.1146 - 1152 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/67258 | - |
dc.description.abstract | For the complete biodegradation of a mixture of benzene, toluene, and p-xylene (BTX), a critical metabolic step that can connect two existing metabolic pathways of aromatic compounds (the tod and the tol pathways) was determined. Toluate-cis-glycol dehydrogenase in the tot pathway was found to attack benzene-cis-glycol, toluene-cis-glycol, and p-xylene-cis-glycol, which are metabolic intermediates of the tod pathway. Based on this observation, a hybrid strain, Pseudomonas putida TB101, was constructed by introduction of the TOL plasmid pWWO into P. putida F39/D, a derivative of P. putida F1, which is unable to transform cis-glycol compounds to corresponding catechols. The metabolic flux of BTX into the tod pathway was redirected to the tot pathway at the level of cis-glycol compounds by the action of toluate-cis-glycol dehydrogenase in P. putida TB101, resulting in the simultaneous mineralization of BTX mixture without accumulation of any metabolic intermediates. The profile of specific degradation rates showed a similar pattern as that of the specific growth rate of the microorganism, and the maximum specific degradation rates of benzene, toluene, and p-xylene were determined to be about 0.27, 0.86, and 2.89 mg/mg biomass/h, respectively. P. putida TB101 is the first reported microorganism that mineralizes BTX mixture simultaneously. (C) 1994 John Wiley & Sons, Inc. | - |
dc.language | English | - |
dc.publisher | JOHN WILEY SONS INC | - |
dc.subject | AEROBIC BIODEGRADATION | - |
dc.subject | AROMATIC-COMPOUNDS | - |
dc.subject | DEGRADATION | - |
dc.subject | OXIDATION | - |
dc.subject | CATECHOL | - |
dc.subject | BACTERIA | - |
dc.subject | AQUIFER | - |
dc.subject | PATHWAY | - |
dc.subject | OPERON | - |
dc.title | METABOLIC ENGINEERING OF PSEUDOMONAS-PUTIDA FOR THE SIMULTANEOUS BIODEGRADATION OF BENZENE, TOLUENE, AND P-XYLENE MIXTURE | - |
dc.type | Article | - |
dc.identifier.wosid | A1994NG86500019 | - |
dc.identifier.scopusid | 2-s2.0-0028434213 | - |
dc.type.rims | ART | - |
dc.citation.volume | 43 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 1146 | - |
dc.citation.endingpage | 1152 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | LEE, JY | - |
dc.contributor.nonIdAuthor | ROH, JR | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | BIODEGRADATION | - |
dc.subject.keywordAuthor | BENZENE | - |
dc.subject.keywordAuthor | TOLUENE | - |
dc.subject.keywordAuthor | P-XYLENE | - |
dc.subject.keywordAuthor | HYBRID STRAIN | - |
dc.subject.keywordPlus | AEROBIC BIODEGRADATION | - |
dc.subject.keywordPlus | AROMATIC-COMPOUNDS | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | CATECHOL | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | AQUIFER | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | OPERON | - |
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